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Expression and Purification of Bioactive High-Purity Recombinant Mouse CXCL14 in Escherichia coli

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Abstract

Mouse CXCL14/BRAK is a monocyte-selective chemokine which is expressed in almost all normal tissues. A flood of reports on its new functions of tumor suppression and fat metabolism modulation has left CXCL14 a potential therapeutic candidate for these diseases. Therefore, a simple accessible method is on demand for large-scale production of recombinant mouse CXCL14 protein for in vivo animal studies. Here, we introduce an efficient method for large-scale production of recombinant mouse CXCL14, by which an 18-mg protein is produced from 2-L Escherichia coli culture with good bioactivity and low level of endotoxin.

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Authors and Affiliations

  1. Shanghai Municipality Key Laboratory of Veterinary Medicine and Biotechnology, School of Agriculture and Biology, Shanghai, People’s Republic of China

    Jingjing Li & Yan Yu

  2. Laboratory of Regeneromics, School of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200240, People’s Republic of China

    Jin Gao, Sunita Sah, Uttam Satyal & Wei Han

  3. General Regeneratives (Shanghai) Limited, Shanghai, People’s Republic of China

    Ruliang Zhang

  4. School of Pharmacy, Shanghai Jiao Tong University, Room 6–202, 800 Dongchuan Road, Minhang District, Shanghai, 200240, People’s Republic of China

    Wei Han

  5. School of Agriculture and Biology, Shanghai Jiao Tong University, Room 3409, 800 Dongchuan Road, Minhang District, Shanghai, 200240, People’s Republic of China

    Yan Yu

Authors
  1. Jingjing Li
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  2. Jin Gao
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  3. Sunita Sah
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  4. Uttam Satyal
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  5. Ruliang Zhang
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  6. Wei Han
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  7. Yan Yu
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Corresponding authors

Correspondence to Wei Han or Yan Yu.

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Li, J., Gao, J., Sah, S. et al. Expression and Purification of Bioactive High-Purity Recombinant Mouse CXCL14 in Escherichia coli . Appl Biochem Biotechnol 164, 1366–1375 (2011). https://doi.org/10.1007/s12010-011-9218-y

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  • DOI: https://doi.org/10.1007/s12010-011-9218-y

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