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d(−)-Lactic Acid Production by Leuconostoc mesenteroides B512 Using Different Carbon and Nitrogen Sources

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Abstract

Sugar concentration from sugarcane juice and yeast autolysate increased lactic acid production more than the other agro-industrial substrates tested. The concentrations of these two components were further optimized using the Plackett–Burman design and response surface method. A second-order polynomial regression model estimated that a maximal lactic acid production of 66.11 g/L would be obtained when the optimal values of sugar and yeast autolysate were 116.9 and 44.25 g/L, respectively. To validate the optimization of the medium composition, studies were carried out using the optimized conditions to confirm the result of the response surface analysis. After 48 h, lactic acid production using the shake-flask method was at 60.2 g/L.

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Acknowledgments

The authors are grateful to Tavolaro Dairy and Corn Products for kindly supplying the whey and corn steep liquor, respectively, the Sta. Lucia plant for supplying sugarcane molasses and sugarcane juice, the Brazilian fostering agency São Paulo Research Foundation—FAPESP, and the National Council for Scientific and Technological Development (CNPq) for the fellowships and financial support.

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Authors and Affiliations

  1. Department of Biochemistry and Microbiology, Univ. Estadual Paulista—UNESP, Biological Sciences Institute, CEP 13506-900, Rio Claro, SP, Brazil

    Luciana Fontes Coelho, Cristian J. Bolner de Lima, Marcela Piassi Bernardo & Jonas Contiero

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  1. Luciana Fontes Coelho
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  2. Cristian J. Bolner de Lima
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  3. Marcela Piassi Bernardo
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  4. Jonas Contiero
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Correspondence to Jonas Contiero.

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Coelho, L.F., de Lima, C.J.B., Bernardo, M.P. et al. d(−)-Lactic Acid Production by Leuconostoc mesenteroides B512 Using Different Carbon and Nitrogen Sources. Appl Biochem Biotechnol 164, 1160–1171 (2011). https://doi.org/10.1007/s12010-011-9202-6

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  • DOI: https://doi.org/10.1007/s12010-011-9202-6

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