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Solvent-Stable Digestive Alkaline Proteinases from Striped Seabream (Lithognathus mormyrus) Viscera: Characteristics, Application in the Deproteinization of Shrimp Waste, and Evaluation in Laundry Commercial Detergents

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Abstract

Alkaline proteases from the viscera of the striped seabream (Lithognathus mormyrus) were extracted and characterized. Interestingly, the crude enzyme was active over a wide range of pH from 6.0 to 11.0, with an optimum pH at the range of 8.0–10.0. In addition, the crude protease was stable over a broad pH range (5.0–12.0). The optimum temperature for enzyme activity was 50 °C. The crude alkaline proteases showed stability towards various surfactants and bleach agents and compatibility with some commercial detergents. It was stable towards several organic solvents and retained more than 50% of its original activity after 30 days of incubation at 30 °C in the presence of 25% (v/v) dimethyl sulfoxide, N,N-dimethylformamide, diethyl ether, and hexane. The crude enzyme extract was also tested for shrimp waste deproteinization in the preparation of chitin. The protein removal with a ratio enzyme/substrate of 10 was about 79%.

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Acknowledgments

This work was funded by the Ministry of Higher Education and Scientific Research, Tunisia.

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Authors and Affiliations

  1. Laboratoire de Génie Enzymatique et de Microbiologie—Ecole Nationale d’Ingénieurs de Sfax, B.P. 1173, 3038, Sfax, Tunisia

    Nedra El-Hadj Ali, Noomen Hmidet, Olfa Ghorbel-Bellaaj, Nahed Fakhfakh-Zouari, Ali Bougatef & Moncef Nasri

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  1. Nedra El-Hadj Ali
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  2. Noomen Hmidet
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  3. Olfa Ghorbel-Bellaaj
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  4. Nahed Fakhfakh-Zouari
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  5. Ali Bougatef
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  6. Moncef Nasri
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Correspondence to Moncef Nasri.

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El-Hadj Ali, N., Hmidet, N., Ghorbel-Bellaaj, O. et al. Solvent-Stable Digestive Alkaline Proteinases from Striped Seabream (Lithognathus mormyrus) Viscera: Characteristics, Application in the Deproteinization of Shrimp Waste, and Evaluation in Laundry Commercial Detergents. Appl Biochem Biotechnol 164, 1096–1110 (2011). https://doi.org/10.1007/s12010-011-9197-z

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