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Exploring Applications of Procerain B, a Novel Protease from Calotropis procera, and Characterization by N-Terminal Sequencing as well as Peptide Mass Fingerprinting

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Abstract

Procerain B is a novel cysteine protease isolated from Calotropis procera by our group and published recently. We have further characterized the enzyme by N-terminal sequencing and peptide mass fingerprinting. Procerain B showed maximum sequence similarity (80%) with Asclepain. Moreover, the characteristic VDWR motif of cysteine proteases is present in procerain B. The N-terminal and peptide mass fingerprinting analysis showed a distinct nature of the enzyme. Various applications of the enzyme were also evaluated. Procerain B is very effective in milk-clotting and may be a potential candidate for this process in the cheese industry. Additionally, the enzyme has potential application as dietary supplement to aid digestion. Effects of various metal ions on milk-clotting activity were also studied. The milk-clotting activity was increased in case of few metals while others have a negative effect. It is worth mentioning that the easy availability of plant material and simple purification method makes industrial production of the enzyme feasible. A protease with easy purification and suitable properties for application is always desired.

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References

  1. Dubey, V. K., Pande, M., Singh, B. K., & Jagannadham, M. V. (2007). African Journal of Biotechnology, 6, 1077–1086.

    CAS  Google Scholar 

  2. Nallamsetty, S., Dubey, V. K., Pande, M., Ambasht, P. K., & Jagannadham, M. V. (2007). Biochimie, 89, 1416–1424.

    Article  CAS  Google Scholar 

  3. Kumar, V. L., & Basu, N. (1994). Journal of Ethnopharmacology, 44, 123–125.

    Article  CAS  Google Scholar 

  4. Arya, S., & Kumar, V. L. (2005). Mediators of inflammation, 4, 228–232.

    Article  Google Scholar 

  5. Sangraula, H., Dewan, S., & Kumar, V. L. (2002). Inflammopharmacology, 9, 257–264.

    Article  Google Scholar 

  6. Dewan, S., Sangraula, H., & Kumar, V. L. (2000). Journal of Ethnopharmacology, 73, 307–311.

    Article  CAS  Google Scholar 

  7. Dewan, S., Kumar, S., & Kumar, V. L. (2000). Antipyretic effect of latex of Calotropis procera. Indian Journal of Pharmacology, 32, 252.

    Google Scholar 

  8. Yesmin, M. N., Uddin, S. N., Mubassara, S., & Akond, M. A. (2008). American-Eurasian Journal of Agricultural and Environmental Science, 4, 550–553.

    Google Scholar 

  9. Dubey, V. K., & Jagannadham, M. V. (2003). Phytochemistry, 62, 1057–1071.

    Article  Google Scholar 

  10. Singh, A. N., Shukla, A. K., Jagannadham, M. V., & Dubey, V. K. (2010). Process Biochemistry, 45, 399–406.

    Article  CAS  Google Scholar 

  11. Berridge, N. J. (1952). The Journal of Dairy Research, 9, 328–329.

    Article  Google Scholar 

  12. Choli, T., Kapp, U., & Wittmann-Liebold, B. (1989). Journal of Chromatography, 476, 59–72.

    Article  CAS  Google Scholar 

  13. Matsudaria, P. (1987). The Journal of Biological Chemistry, 262, 10035–10038.

    Google Scholar 

  14. Katayama, H., Nagasu, T., & Oda, Y. (2001). Rapid Communications in Mass Spectrometry, 15, 416–421.

    Article  Google Scholar 

  15. Rawlings, N.M. & Barrett, A.J. (2004). In: Barrett, A. J., Rawlings, N. M., & Woessner, J. F. (Eds.), Handbook of proteolytic enzymes, 2nd edition, vol. II (pp. 1051–1071). London: Elsevier Academic Press.

  16. Obregón, W. D., Liggieri, C. S., Trejo, S. A., Avilés, F. X., Vairo-Cavalli, S. E., & Priolo, N. S. (2009). Biochimie, 91, 1457–1464.

    Article  Google Scholar 

  17. Trejo, S. A., López, L. M. I., Caffini, N. O., Natalucci, C. L., Canals, F., & Avilés, F. (2009). Planta, 230, 319–328.

    Article  CAS  Google Scholar 

  18. Lynn, K. R., Yaguchi, M., & Roy, C. (1980). Biochimica et Biophysica Acta, 624, 579–580.

    CAS  Google Scholar 

  19. Taylor, M. A., Baker, K. C., Briggs, G. S., Connerton, I. F., Cummings, N. J., Pratt, K. A., et al. (1995). Protein Engineering, 8, 59–62.

    Article  CAS  Google Scholar 

  20. Cavalli, S. V., Cortadi, A., Arribere, M. C., Conforti, P., Caffini, N. O., & Priolo, N. (2001). Biological Chemistry, 382, 879–883.

    Article  CAS  Google Scholar 

  21. Mitchel, R. E. J., Chaiken, I. M., & Smith, E. L. (1970). The Journal of Biological Chemistry, 245, 3485–3492.

    CAS  Google Scholar 

  22. Revell, D. F., Cummings, N. J., Baker, K. C., Collins, M. E., Taylor, M. A., Sumner, I. G., et al. (1993). Gene, 127, 221–225.

    Article  CAS  Google Scholar 

  23. Sequeiros, C., Torres, M. J., Trejo, S. A., Esteves, J. L., Natalucci, C. L., & López, L. M. I. (2005). The Protein Journal, 24, 445–453.

    Article  CAS  Google Scholar 

  24. Kundu, S., Sundd, M., & Jagannadham, M. V. (2000). Journal of Agricultural and Food Chemistry, 48, 171–179.

    Article  CAS  Google Scholar 

  25. Sundd, M., Kundu, S., Pal, G. P., & Medicherla, J. V. (1998). Bioscience, Biotechnology, and Biochemistry, 62, 1947–1955.

    Article  CAS  Google Scholar 

  26. Patel, B. K., & Jagannadham, M. V. (2003). Journal of Agricultural and Food Chemistry, 51, 6326–6334.

    Article  CAS  Google Scholar 

  27. Goto, K., Takahashi, N., & Murachi, T. (1980). International Journal of Peptide Research, 15, 335–341.

    Article  CAS  Google Scholar 

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Acknowledgments

Research fellowship to ANS by the Indian Institute of Technology Guwahati is acknowledged. Authors acknowledge Proteomics Facility and Protein Sequencing facility of the Indian Institute of Science for help in the experiments.

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  1. Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, 781039, Assam, India

    Abhay Narayan Singh & Vikash Kumar Dubey

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  1. Abhay Narayan Singh
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  2. Vikash Kumar Dubey
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Correspondence to Vikash Kumar Dubey.

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Singh, A.N., Dubey, V.K. Exploring Applications of Procerain B, a Novel Protease from Calotropis procera, and Characterization by N-Terminal Sequencing as well as Peptide Mass Fingerprinting. Appl Biochem Biotechnol 164, 573–580 (2011). https://doi.org/10.1007/s12010-011-9158-6

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  • DOI: https://doi.org/10.1007/s12010-011-9158-6

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