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Comparison of the Properties of Lipase Immobilized onto Mesoporous Resins by Different Methods

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Abstract

Genipin, a natural cross-linking agent, was used for the immobilization of lipase from Candida sp. 99-125 by cross-linking to two kinds of mesoporous resins. Under optimum conditions, the activity recovery of immobilized lipase on resin NKA-9 could reach up to 96.99% when the genipin concentration was 0.5%, and it could reach up to 86.18% for S-8 with a genipin concentration of 0.25%. Compared with using glutaraldehyde as a cross-linking agent, the immobilized lipase using genipin showed better pH and thermal stability, storage stability, and reusability. The residual activity of immobilized lipase using genipin as cross-linker remained more than 60% of its initial activity after six hydrolytic cycles, whereas only about 35% activity remained by using glutaraldehyde as cross-linker.

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Acknowledgments

This work was supported by the Natural Science Foundation of Hebei Province (B2010000035, B2008000028) and the Natural Science Foundation of Tianjin (08JCYBJC02400).

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Authors and Affiliations

  1. Department of Bioengineering, School of Chemical Engineering, Hebei University of Technology, Tianjin, 300130, China

    Wenqin Wang, Yanjun Jiang, Liya Zhou & Jing Gao

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  1. Wenqin Wang
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  2. Yanjun Jiang
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  3. Liya Zhou
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  4. Jing Gao
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Correspondence to Jing Gao.

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Wang, W., Jiang, Y., Zhou, L. et al. Comparison of the Properties of Lipase Immobilized onto Mesoporous Resins by Different Methods. Appl Biochem Biotechnol 164, 561–572 (2011). https://doi.org/10.1007/s12010-010-9157-z

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