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Study of the Alkyl Chain Length on Laccase Stability and Enzymatic Kinetic with Imidazolium Ionic Liquids

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Abstract

The activity and stability of laccase and their kinetic mechanisms in water soluble ionic liquids (ILs): 1-butyl-3-methyl imidazolium chloride [C4mim][Cl], 1-octyl-3-methyl imidazolium chloride [C8mim][Cl], and 1-decyl-3-methyl imidazolium chloride [C10mim][Cl] were investigated. The results show that an IL concentration up to 10% is satisfactory for initial laccase activity at pH 9.0. The laccase stability was well maintained in [C4mim][Cl] IL when compared to the control. The inactivation of laccase increases with the length of the alkyl chain in the IL: [C10mim][Cl] > [C8mim][Cl] > [C4mim][Cl]. The kinetic studies in the presence of ABTS as substrate allowed calculating the Michaelis–Menten parameters. Among the ILs, [C4mim][Cl] was the suitable choice attending to laccase activity and stability. Alkyl chains in the ions of ILs have a deactivating effect on laccase, which increases strongly with the length of the alkyl chain.

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Authors and Affiliations

  1. LSRE–Laboratory of Separation and Reaction Engineering, Associate Laboratory LSRE/LCM, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias s/n, 4200-465, Porto, Portugal

    Oscar Rodríguez, Raquel O. Cristóvão, Ana P. M. Tavares & Eugénia A. Macedo

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  1. Oscar Rodríguez
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  2. Raquel O. Cristóvão
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  3. Ana P. M. Tavares
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  4. Eugénia A. Macedo
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Correspondence to Eugénia A. Macedo.

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Rodríguez, O., Cristóvão, R.O., Tavares, A.P.M. et al. Study of the Alkyl Chain Length on Laccase Stability and Enzymatic Kinetic with Imidazolium Ionic Liquids. Appl Biochem Biotechnol 164, 524–533 (2011). https://doi.org/10.1007/s12010-010-9154-2

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  • DOI: https://doi.org/10.1007/s12010-010-9154-2

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