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Effects of Pluronic F68 on Manganese Peroxidase Production by Pelletized Phanerochaete chrysosporium

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Abstract

In this study, a new process was developed for manganese peroxidase (MnP) production by Phanerochaete chrysosporium under an agitated and aerated cultivation condition. It was found that change of the inoculum from spore suspension to pellets resulted in enhanced MnP production of 200 U/L in rotated shake flasks. Several additives, including Pluronic F68, Tween 80, and PEG8000, significantly increased the enzyme production. With an optimal concentration in 125 mL flasks, Pluronic F68 increased MnP productivity by 180%. Moreover, successful enzyme production was achieved in a 5-L fermentor at an agitation speed of 300 rpm with the addition of 0.1% Pluronic F68.

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Acknowledgement

The research was partially supported by the Agricultural Research Center of Washington State University and Shanghai Municipal Natural Science Foundation (09ZR1407800).

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Authors and Affiliations

  1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China

    Zhi-min Li

  2. Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI, 48824, USA

    Yan Liu

  3. Department of Biological Systems Engineering, Washington State University, L.J. Smith 213, Pullman, WA, 99164, USA

    Zhan-you Chi & Shu-lin Chen

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  1. Zhi-min Li
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  2. Yan Liu
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  4. Shu-lin Chen
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Correspondence to Shu-lin Chen.

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Li, Zm., Liu, Y., Chi, Zy. et al. Effects of Pluronic F68 on Manganese Peroxidase Production by Pelletized Phanerochaete chrysosporium . Appl Biochem Biotechnol 164, 487–496 (2011). https://doi.org/10.1007/s12010-010-9150-6

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  • DOI: https://doi.org/10.1007/s12010-010-9150-6

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