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Effect of Biosurfactants on Laccase Production and Phenol Biodegradation in Solid-State Fermentation

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Abstract

The effects of two biosurfactants, tea saponin (TS) and rhamnolipid (RL), on the production of laccase and the degradation of phenol by P. simplicissimum were investigated in solid-state fermentation consisting of rice straw, rice bran, and sawdust. Firstly, the effects of phenol on the fermentation process were studied in the absence of surfactants. Then, a phenol concentration of 3 mg/g in the fermentation was selected for detailed research with the addition of biosurfactants. The results showed that TS and RL at different concentrations had stimulative effects on the enzyme activity of laccase. The highest laccase activities during the fermentation were enhanced by 163.7%, 68.2%, and 23.3% by TS at concentrations of 0.02%, 0.06%, and 0.10%, respectively. As a result of the enhanced laccase activity, the efficiency of phenol degradation was also improved by both biosurfactants. RL caused a significant increase of fungal biomass in the early stage of the fermentation, while TS had an inhibitory effect in the whole process. These results indicated that RL could mitigate the negative effects of phenol on fungal growth and consequently improve laccase production and phenol degradation. TS was potentially applicable to phenol-polluted solid-state fermentation.

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Acknowledgments

This research was financially supported by the National Natural Science Foundation of China (No. 50978087), the Hunan Province Science and Technology Project (2009 FJ-1010-4), the Innovative Research Team in University (IRT0719), and the Hunan University Graduate Education Innovation Project (531107011019).

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Authors and Affiliations

  1. College of Environmental Science and Engineering, Hunan University, Changsha, 410082, People’s Republic of China

    Mei-Fang Zhou, Xing-Zhong Yuan, Hua Zhong, Zhi-Feng Liu, Hui Li, Li-Li Jiang & Guang-Ming Zeng

  2. Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha, 410082, People’s Republic of China

    Mei-Fang Zhou, Xing-Zhong Yuan, Hua Zhong, Zhi-Feng Liu, Hui Li, Li-Li Jiang & Guang-Ming Zeng

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  1. Mei-Fang Zhou
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Correspondence to Xing-Zhong Yuan.

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Zhou, MF., Yuan, XZ., Zhong, H. et al. Effect of Biosurfactants on Laccase Production and Phenol Biodegradation in Solid-State Fermentation. Appl Biochem Biotechnol 164, 103–114 (2011). https://doi.org/10.1007/s12010-010-9118-6

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  • DOI: https://doi.org/10.1007/s12010-010-9118-6

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