Hydrolytic Enzyme of Cellulose for Complex Formulation Applied Research
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To improve the enzymatic hydrolytic efficiency and reduce the supplementation of enzymes, the mixture designed experimental approach was used to optimize the composition of enzyme mixture and promote the hydrolysis of ball-milled corn stover. From the experimental results, a synergistic effect was found when combinations of the three enzymes, two kinds of cellulases and a kind of xylanase, were used. The optimal hydrolysis of pretreated corn stover accorded with enzymes activity ration of FPU/CMCase/β-glucosidase/xylanase = 4.4:1:75:829, and the hydrolysis efficiency of corn stover increased significantly compared with using individual enzyme. The results indicated that the mixture design experiment could be an effective tool for optimized enzyme mixture for lignocellulose hydrolysis.
KeywordsExperimental mixture design Cellulose Complex formulation Cellulase Xylanase
This work was financially supported by China Petroleum & Chemical Corporation (No. 207035), National Natural Science Foundation of China (No. 20876078), the Key Program of National Natural Science Foundation of China (No. 20936002), United Foundation of NSFC and Guangdong Province (No. U0733001), National Hi-tech Research and Development Program of China (No. 2009AA02Z08) and National Basic Research Program of China (No. 2007CB707805).
- 15.Nidetzky, B., Steiner, W., Hayn, M., & Claeyssens, M. (1994). Cellulose hydrolysis by the cellulases from Trichoderma reesei: a new model for synergistic interaction. Biochemical Journal, 298, 705–710.Google Scholar
- 25.Lin, Z., Huang, H., Zhang, H., Yan, L., Chen, J., Jin, Q., et al. (2009). Optimization of process parameters of ball milling pretreatment of corn stalk. Transactions of the Chinese Society of Agricultural Engineering, 25(3), 202–204.Google Scholar
- 27.NREL. National Renewable Energy Laboratory standard methods of Laboratory Analytical Procedure (LAP) (2009). Available from: < http://www.nrel.gov/biomass/analytical_procedures.html >
- 33.Sanchez, M. M., Irwin, D. C., Pastor, F. I. J., Wilson, D. B., & Diaz, P. (2004). Synergistic activity of Paenibacillus sp BP-23 cellobiohydrolase Ce148C in association with the contiguous endoglucanase Ce19B and with endoor exo-acting glucanases from Thermobifida fusca. Biotechnology and Bioengineering, 87(2), 161–169.CrossRefGoogle Scholar