Applied Biochemistry and Biotechnology

, Volume 164, Issue 1, pp 23–33 | Cite as

Hydrolytic Enzyme of Cellulose for Complex Formulation Applied Research

  • Zeng-Xiang Lin
  • Hong-Man Zhang
  • Xiao-Jun Ji
  • Jing-Wen Chen
  • He HuangEmail author


To improve the enzymatic hydrolytic efficiency and reduce the supplementation of enzymes, the mixture designed experimental approach was used to optimize the composition of enzyme mixture and promote the hydrolysis of ball-milled corn stover. From the experimental results, a synergistic effect was found when combinations of the three enzymes, two kinds of cellulases and a kind of xylanase, were used. The optimal hydrolysis of pretreated corn stover accorded with enzymes activity ration of FPU/CMCase/β-glucosidase/xylanase = 4.4:1:75:829, and the hydrolysis efficiency of corn stover increased significantly compared with using individual enzyme. The results indicated that the mixture design experiment could be an effective tool for optimized enzyme mixture for lignocellulose hydrolysis.


Experimental mixture design Cellulose Complex formulation Cellulase Xylanase 



This work was financially supported by China Petroleum & Chemical Corporation (No. 207035), National Natural Science Foundation of China (No. 20876078), the Key Program of National Natural Science Foundation of China (No. 20936002), United Foundation of NSFC and Guangdong Province (No. U0733001), National Hi-tech Research and Development Program of China (No. 2009AA02Z08) and National Basic Research Program of China (No. 2007CB707805).


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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Zeng-Xiang Lin
    • 1
  • Hong-Man Zhang
    • 2
  • Xiao-Jun Ji
    • 1
  • Jing-Wen Chen
    • 1
  • He Huang
    • 1
    Email author
  1. 1.State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical EngineeringNanjing University of TechnologyNanjingPeople’s Republic of China
  2. 2.Department of Applied ChemistryNanjing University of TechnologyNanjingPeople’s Republic of China

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