Abstract
After screening with 0.1% esculoside and 0.03% FeCl3, we identified from rotten wood a fungal isolate HML0366 that produces high amount of β-glucosidase. Phenotypic and rDNA internal transcribed spacer sequence analyses indicated that the isolate belongs to Aspergillus oryzae. The β-glucosidase produced by HML0366 had an activity of 128 U/g. high performance liquid chromatography analysis also demonstrated a high transglycosylation activity of the crude enzyme. The β-glucosidase was stable between pH 4–10 at 60 °C. A gentiobiose yield of 30.86 g/L was achieved within 72 h of the enzymatic reaction at pH 5 and 55 °C using 50% glucose as the substrate. For the first time, we report here the isolation of an A. oryzae strain producing β-glucosidase with high hydrolytic activities. The crude enzyme has a high transglycosylation activity, which enables the enzymatic synthesis of gentiooligosaccharides.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (grant no. 20066001), the Natural Science Foundation of Guangxi Province of China (grant no. 2010GXNSFA013103), and the Academy-Level Key Research Projects of Hechi University (grant no. 2009YAZ-N003).
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Yongling Qin, Yunkai Zhang, and Haiyan He contributed equally to this work.
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Qin, Y., Zhang, Y., He, H. et al. Screening and Identification of a Fungal β-Glucosidase and the Enzymatic Synthesis of Gentiooligosaccharide. Appl Biochem Biotechnol 163, 1012–1019 (2011). https://doi.org/10.1007/s12010-010-9105-y
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DOI: https://doi.org/10.1007/s12010-010-9105-y