Abstract
An efficient tissue culture technology has been designed for mass multiplication of Nyctanthes arbor-tristis L. by preculturing nodal explants in thidiazuron (TDZ)-supplemented liquid Murashige and Skoog (MS) media. Direct inoculation of nodal segments on semi-solid MS medium augmented with various concentrations of TDZ (0.1 to 0.9 μM) produced shoots but with low regeneration response and few shoots per explant. Hence, nodal explants were pretreated with greater concentrations of TDZ (5 to 100 μM) in liquid MS media for different durations (4, 8, 12, and 16 days) with the aim of improving shoot regeneration response from cultured explants. After pretreatment, explants were transferred to agar-solidified hormone-free MS medium. Best response in terms of percent regeneration (94%), number of shoots per explant (20.00 ± 1.15), and greatest shoot length (7.23 ± 0.83 cm) were obtained with nodal segments pretreated in75 μM TDZ for 8 days. Similarly, root induction was obtained from pulse-treated microshoots for 24 h with 200 μM indole-3-butyric acid (IBA) followed by their transfer to 1/2 MS medium which produced an average of 5.50 ± 0.92 roots per microshoot. The rooted plantlets were transplanted to soil with 80% success rate.
Similar content being viewed by others
Abbreviations
- IBA:
-
Indole-3-butyric acid
- MS:
-
Murashige and Skoog medium
- TDZ:
-
Thidiazuron
References
Iyer, R. I., Mathuram, V., & Gopinath, P. M. (1998). Current Science, 74, 243–246.
Anonymous (2001). The wealth Of India: a dictionary of Indian raw materials and industrial products, vol VII: N–Pe (pp. 69–70). New Delhi: Publications and Information Directorate CSIR.
Hukkeri Kusum, A. S., Sureban, R. R., Gopalakrishna, B., Byahatti, V. V., & Rajendra, S. V. (2006). Indian Journal of Pharmaceutical Sciences, 68, 542–543.
Rathee, J. S., Hassarajani, S. A., & Chattopadhyay, S. (2007). Food Chemistry, 103, 1350–1357.
Priya, K., & Ganjewala, D. (2007). Research Journal of Phytochemistry, 1, 61–67.
Nanu, R., Raghuveer, I., Chitme, H. R., & Chandra, R. (2008). Pharmacognosy Magazine, 4, 335–340.
Thapliyal, R. C., & Naithani, K. C. (1996). Seed Science Technology, 24, 67–73.
Siddique, I., Anis, M., & Jahan, A. A. (2006). World Journal of Agricultural Sciences, 2, 188–192.
Rout, G. R., Mahato, A., & Senapati, S. K. (2007). Horticultural Science (Prague.), 34, 84–89.
Rout, G. R., Mahato, A., & Senapati, S. K. (2008). Biologia Plantarum, 52, 521–524.
Huetteman, C. A., & Preece, J. E. (1993). Plant Cell, Tissue and Organ Culture, 33, 105–119.
Faisal, M., Ahmad, N., & Anis, M. (2005). Plant Cell, Tissue and Organ Culture, 80, 187–190.
Shi, X. L., Han, H. P., Shi, W. L., & Li, Y. X. (2006). Journal of Integrative Plant Biology, 48, 1185–1189.
Husain, M. K., Anis, M., & Shahzad, A. (2007). In Vitro Cellular Developmental Biology of Plants, 43, 59–64.
Murashige, T., & Skoog, F. (1962). Physiologiae Plantarum, 15, 473–497.
Siddique, I., & Anis, M. (2007). Biologia Plantarum, 51, 787–790.
Jahan, A. A., & Anis, M. (2009). Acta Physiologiae Plantarum, 31, 133–138.
Sudha, G. C., & Seeni, S. (1994). Plant Cell Reports, 10, 67–70.
Pattnaik, S. K., & Chand, P. K. (1996). Plant Cell Reports, 15, 846–850.
Thomas, T. D. (2003). Biologia Plantarum, 46, 529–533.
Siddique, I., & Anis, M. (2007). Acta Physiologiae Plantarum, 29, 233–238.
Kintzios, S., Sereti, E., Bluchos, P., Drossopoulos, J. B., Kitsaki, C. K., & Liopa-Tsakalidis, A. (2002). Plant Cell Reports, 21, 1–8.
Shan, Z., Raemakers, K., Tzitzikas, E. N., Ma, Z., & Visser, R. G. F. (2005). Plant Cell Reports, 24, 507–512.
D’ Onofrio, C., & Morini, S. (2006). Scientia Horticulturae, 107, 194–199.
Singh, S. K., & Syamal, M. M. (2001). Scientia Horticulturae, 91, 169–177.
Prathanturarug, S., Soonthorncharennon, N., Chuakul, W., Phiadee, Y., & Saralamp, P. (2005). Plant Cell, Tissue and Organ Culture, 80, 347–351.
Thomas, T. D. (2007). Acta Physiologiae Plantarum, 29, 455–461.
Hare, P. D., & Van Staden, J. (1994). Plant and Cell Physiology, 35, 1121–1125.
Murthy, B. N. S., Murch, S. J., & Saxena, P. K. (1998). In Vitro Cellular Developmental Biology of Plants, 34, 267–275.
Magioli, C., Roacha, A. P. M., de Oliveira, D. E., & Mansur, E. (1998). Plant Cell Reports, 17, 661–663.
Goldfrab, B., Howe, G. T., Bailey, L. M., Straues, S. H., & Zaerr, J. B. (1991). Plant Cell Reports, 10, 156–160.
Madhulata, P., Anbalagan, M., Jayachanran, S., & Sakthirel, N. (2004). Plant Cell, Tissue and Organ Culture, 76, 189–191.
Murch, S. J., Choffe, K. L., Victor, J. M. R., Slimmon, T. Y., Krishnaraj, S., & Saxena, P. K. (2000). Plant Cell Reports, 19, 576–581.
Mroginiski, E., Rey, H. Y., Gonzalez, A. M., & Mroginiski, L. A. (2004). Plant Growth Regulation, 23, 129–134.
Feyissa, T., Welander, M., & Negash, L. (2005). Plant Cell, Tissue and Organ Culture, 80, 119–127.
Acknowledgement
The authors gratefully acknowledge the Department of Science and Technology and the University Grants Commission, Government of India, New Delhi for providing research support under DST-FIST (2005) and UGC-DRS (2009) programs.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Jahan, A.A., Anis, M. & Aref, I.M. Preconditioning of Axillary Buds in Thidiazuron-Supplemented Liquid Media Improves In Vitro Shoot Multiplication in Nyctanthes arbor-tristis L.. Appl Biochem Biotechnol 163, 851–859 (2011). https://doi.org/10.1007/s12010-010-9089-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12010-010-9089-7