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Preconditioning of Axillary Buds in Thidiazuron-Supplemented Liquid Media Improves In Vitro Shoot Multiplication in Nyctanthes arbor-tristis L.

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Abstract

An efficient tissue culture technology has been designed for mass multiplication of Nyctanthes arbor-tristis L. by preculturing nodal explants in thidiazuron (TDZ)-supplemented liquid Murashige and Skoog (MS) media. Direct inoculation of nodal segments on semi-solid MS medium augmented with various concentrations of TDZ (0.1 to 0.9 μM) produced shoots but with low regeneration response and few shoots per explant. Hence, nodal explants were pretreated with greater concentrations of TDZ (5 to 100 μM) in liquid MS media for different durations (4, 8, 12, and 16 days) with the aim of improving shoot regeneration response from cultured explants. After pretreatment, explants were transferred to agar-solidified hormone-free MS medium. Best response in terms of percent regeneration (94%), number of shoots per explant (20.00 ± 1.15), and greatest shoot length (7.23 ± 0.83 cm) were obtained with nodal segments pretreated in75 μM TDZ for 8 days. Similarly, root induction was obtained from pulse-treated microshoots for 24 h with 200 μM indole-3-butyric acid (IBA) followed by their transfer to 1/2 MS medium which produced an average of 5.50 ± 0.92 roots per microshoot. The rooted plantlets were transplanted to soil with 80% success rate.

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Abbreviations

IBA:

Indole-3-butyric acid

MS:

Murashige and Skoog medium

TDZ:

Thidiazuron

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Acknowledgement

The authors gratefully acknowledge the Department of Science and Technology and the University Grants Commission, Government of India, New Delhi for providing research support under DST-FIST (2005) and UGC-DRS (2009) programs.

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Correspondence to M. Anis.

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Jahan, A.A., Anis, M. & Aref, I.M. Preconditioning of Axillary Buds in Thidiazuron-Supplemented Liquid Media Improves In Vitro Shoot Multiplication in Nyctanthes arbor-tristis L.. Appl Biochem Biotechnol 163, 851–859 (2011). https://doi.org/10.1007/s12010-010-9089-7

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