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Extracellular Production and Characterization of Two Streptomyces l-Asparaginases

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Abstract

l-Asparaginase (ASNase) has proved its use in medical and food industries. Sequence-based screening showed the thermophilic Streptomyces strain Streptomyces thermoluteus subsp. fuscus NBRC 14270 (14270 ASNase) to positive against predicted ASNase primary sequences. The 14270 ASNase gene and four l-asparaginase genes from Streptomyces coelicolor, Streptomyces avermitilis, and Streptomyces griseus (SGR ASNase) were expressed in Streptomyces lividans using a hyperexpression vector: pTONA5a. Among those genes, only 14270 ASNase and SGR ASNase were successful for overexpression and detected in culture supernatants without an artificial signal peptide. Comparison of the two Streptomyces enzymes described above demonstrated that 14270 ASNase was superior to SGR ASNase in terms of optimum temperature, thermal stability, and pH stability.

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Acknowledgment

We thank Y. Nishimoto for assistance in the construction of expression plasmids.

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Authors and Affiliations

  1. Okayama Prefectural Technology Center for Agriculture, Forestry and Fisheries, Research Institute for Biological Sciences (RIBS), Okayama, 7549-1 Kibichuo-cho, Kaga-gun, Okayama, 716-1241, Japan

    Tadashi Hatanaka, Hirokazu Usuki, Yukihiro Yamamoto, Yuya Kumagai, Akihiro Yamasato & Takafumi Mukaihara

  2. Japan Society for the Promotion of Science (JSPS), Tokyo, Japan

    Hirokazu Usuki

  3. Department of Agricultural, Biological, and Environmental Sciences, Faculty of Agriculture, Tottori University, 4-101 Koyama-minami, Tottori, 680-8553, Japan

    Jiro Arima

  4. Department of Biomaterials, Field of Tissue Engineering, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawara-cho Shogoin, Sakyo-ku, Kyoto, 606-8507, Japan

    Yoshiko Uesugi

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  1. Tadashi Hatanaka
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  2. Hirokazu Usuki
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  3. Jiro Arima
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  4. Yoshiko Uesugi
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  5. Yukihiro Yamamoto
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  6. Yuya Kumagai
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  7. Akihiro Yamasato
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  8. Takafumi Mukaihara
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Correspondence to Tadashi Hatanaka.

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Hatanaka, T., Usuki, H., Arima, J. et al. Extracellular Production and Characterization of Two Streptomyces l-Asparaginases. Appl Biochem Biotechnol 163, 836–844 (2011). https://doi.org/10.1007/s12010-010-9087-9

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  • DOI: https://doi.org/10.1007/s12010-010-9087-9

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