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Poly-β-hydroxybutyrate and Exopolysaccharide Biosynthesis by Bacterial Isolates from Pigeonpea [Cajanus cajan (L.) Millsp] Root Nodules

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Abstract

The bacterial strains that are able to produce biopolymers that are applied in industrial sectors present a source of renewable resources. Some microorganisms are already applied at several industrial sectors, but the prospecting of new microbes must bring microorganisms that are feasible to produce interesting biopolymers more efficiently and in cheaper conditions. Among the biopolymers applied industrially, polyhydroxybutyrate (PHB) and exopolysaccharides (EPS) stand out because of its applications, mainly in biodegradable plastic production and in food industry, respectively. In this context, the capacity of bacteria isolated from pigeonpea root nodules to produce EPS and PHB was evaluated, as well as the cultural characterization of these isolates. Among the 38 isolates evaluated, the majority presented fast growth and ability to acidify the culture media. Regarding the biopolymer production, five isolates produced more than 10 mg PHB per liter of culture medium. Six EPS producing bacteria achieved more than 200 mg EPS per liter of culture medium. Evaluating different carbon sources, the PHB productivity of the isolate 24.6b reached 69% of cell dry weight when cultured with starch as sole carbon source, and the isolate 8.1c synthesized 53% PHB in dry cell biomass and more than 1.3 g L−1 of EPS when grown using xylose as sole carbon source.

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Acknowledgements

The authors thanks Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Embrapa Agrobiologia, and Curso de Pós-graduação em Agronomia-Ciência do Solo (CPGA-CS) of Universidade Federal Rural do Rio de Janeiro, which provided financial and structural support.

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Authors and Affiliations

  1. Instituto de Agronomia, Departamento de Solos, Universidade Federal Rural do Rio de Janeiro, BR 465, km 7–23890-000, Seropédica, RJ, Brasil

    Paulo Ivan Fernandes Júnior

  2. Instituto de Tecnologia, Departamento de Engenharia Química, Universidade Federal Rural do Rio de Janeiro, BR 465, km 7–23890-000, Seropédica, RJ, Brasil

    Paulo Jansen de Oliveira

  3. Embrapa Agrobiologia, BR 465, km 7–23890-000, Seropédica, RJ, Brasil

    Norma Gouvêa Rumjanek & Gustavo Ribeiro Xavier

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  1. Paulo Ivan Fernandes Júnior
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  2. Paulo Jansen de Oliveira
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  3. Norma Gouvêa Rumjanek
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  4. Gustavo Ribeiro Xavier
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Correspondence to Paulo Ivan Fernandes Júnior.

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Fernandes Júnior, P.I., de Oliveira, P.J., Rumjanek, N.G. et al. Poly-β-hydroxybutyrate and Exopolysaccharide Biosynthesis by Bacterial Isolates from Pigeonpea [Cajanus cajan (L.) Millsp] Root Nodules. Appl Biochem Biotechnol 163, 473–484 (2011). https://doi.org/10.1007/s12010-010-9055-4

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  • DOI: https://doi.org/10.1007/s12010-010-9055-4

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