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Applied Biochemistry and Biotechnology

, Volume 163, Issue 1, pp 112–126 | Cite as

Identification of a Keratinase-Producing Bacterial Strain and Enzymatic Study for Its Improvement on Shrink Resistance and Tensile Strength of Wool- and Polyester-Blended Fabric

  • Shao-Bo Cai
  • Zheng-Hua Huang
  • Xing-Qun Zhang
  • Zhang-Jun CaoEmail author
  • Mei-Hua Zhou
  • Feng Hong
Article

Abstract

A wool-degrading bacterium was isolated from decomposition wool fabrics in China. The strain, named 3096-4, showed excellent capability of removing cuticle layer of wool fibers, as demonstrated by removing cuticle layer completely within 48 h. According to the phenotypic characteristics and 16S rRNA profile, the isolate was classified as Pseudomonas. Bacteria growth and keratinase activity of the isolate were determined during cultivation on raw wool at different temperatures, initial pH, and rotation speed using orthogonal matrix method. Maximum growth and keratinase activity of the bacterium were observed under the condition including 30 °C, initial pH 7.6, and rotational speeds 160 rpm. The keratinase-containing crude enzyme prepared from 3096-4 was evaluated in the treatment of wool fabrics. The optimal condition of our enzymatic improvement of shrink resistance was the combination of 30 °C, initial pH 7.6, and rotation speeds 160 rpm. After the optimized treatment, the wool fabrics felting shrink was 4.1% at 6 h, and textile strength was not lost.

Keywords

Pseudomonas Keratinase Wool fabrics Shrink resistance 

Notes

Acknowledgments

This work was partial supported by Ph.D. program foundation of Ministry of Education of China (No.20090075110007) and partially supported by Chinese Universities Scientific Fund. We are grateful to Zhiyong Deng for critical reading of the manuscript.

Supplementary material

12010_2010_9021_MOESM1_ESM.doc (137 kb)
ESM 1 (DOC 137 kb)

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Shao-Bo Cai
    • 1
    • 2
  • Zheng-Hua Huang
    • 1
    • 2
  • Xing-Qun Zhang
    • 2
  • Zhang-Jun Cao
    • 1
    • 2
    Email author
  • Mei-Hua Zhou
    • 3
  • Feng Hong
    • 1
  1. 1.Group Microbiological Engineering & Industrial Biotechnology, College of Chemistry, Chemical Engineering and BiotechnologyDonghua UniversityShanghaiChina
  2. 2.Key Laboratory of Science & Technology of Eco-Textile, Ministry of EducationDonghua UniversityShanghaiChina
  3. 3.College of Environmental Science & EngineeringDonghua UniversityShanghaiChina

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