Enzyme Production by Solid Substrate Fermentation of Pleurotus ostreatus and Trametes versicolor on Tomato Pomace
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A process of solid state fermentation (SSF) on tomato pomace was developed with the white-rot fungi Pleurotus ostreatus and Trametes versicolor, using sorghum stalks as support. Operative parameters (humidity, water activity, and size of substrate particles) guaranteeing a good colonization of tomato pomace by both fungi were defined and conditions for production at high titers of the industrially relevant enzymes laccase, xylanase and protease were identified. Significant laccase activity levels (up to 36 U g−1 dry matter) were achieved without any optimization of culture conditions, neither by nutrient addition nor by O2 enrichment. Furthermore, protease activity levels up to 34,000 U g−1 dry matter were achieved, being higher than those reported for the fungi typically considered as the best protease producers such as Aspergillus strains. Moreover, as one of the most significant results of this study, analysis of P. ostreatus tomato SSF samples by zymogram revealed two bands with laccase activity which had not been detected so far.
KeywordsFungi Solid state fermentation Laccase Xylanase Protease
This work was supported by grants from the Ministero dell’Università e della Ricerca Scientifica (Progetti di Rilevante Interesse Nazionale, PRIN), and from the Ministero Degli Affari Esteri di Intesa con il Ministero dell’Università e della Ricerca (Progetti di ricerca di base e tecnologica approvati nei protocolli di cooperazione scientifica e tecnologica bilaterale come previsto dal protocollo bilaterale tra Italia e Turchia).
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