Enantioselective Resolution of γ-Lactam by a Whole Cell of Microbacterium hydrocarbonoxydans (L29-9) Immobilized in Polymer of PVA–Alginate–Boric Acid
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Using immobilized cells of a novel strain of Microbacterium hydrocarbonoxydans L29-9 in polymers of polyvinyl alcohol (PVA)–alginate–boric acid, enantioselective resolution of racemic γ-lactam to produce (−)γ-lactam was successfully carried out. A 6:1 ratio of PVA:sodium alginate not only prevented agglomeration of the matrix but also produced beads with high gel strength. The optimum biotransformation conditions were 1 g/L substrate, pH 7.0, reaction temperature of 30 °C, and reaction time of 3 h. After every two cycles, the immobilized cell beads were separated and immersed in 0.5 mM KCl solution at 4 °C for preservation. At optimum conditions, the enantiomeric excess and the yield of (−)γ-lactam were >99% and 34%, respectively. The beads showed a slight decrease in the enantiomeric excess when re-used up to 14 cycles (the enantioselectivity of the immobilized cells decreased slightly after 14 cycles of usage).
KeywordsAbacavir Enantioselective resolution γ-Lactam Immobilized Microbacterium hydrocarbonoxydans cells Polyvinyl alcohol
This work was supported by the 863 program (2006AA02Z250); the 973 program (2004CB719606); the Ministry of Science and Technology, China; the Open Fund of State Key Laboratory of Microbial Resources, the Institute of Microbiology, the Chinese Academy of Sciences (SKLMR-08060).
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