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Applied Biochemistry and Biotechnology

, Volume 162, Issue 8, pp 2232–2243 | Cite as

Application of Supermacroporous Monolithic Hydrophobic Cryogel in Capturing of Albumin

  • Nesibe Avcibaşi
  • Murat Uygun
  • M. Emin Çorman
  • Sinan AkgölEmail author
  • Adil Denizli
Article

Abstract

Supermacroporous poly{2-hydroxyethyl methacrylate-co-[N,N-bis(2,6-diisopropylphenyl)-perylene-3,4,9,10-tetracarboxylic diimide]} [poly(HEMA-co-DIPPER)] monolithic cryogel column was prepared by radical cryocopolymerization of HEMA with DIPPER as functional comonomer and N,N′-methylene-bisacrylamide (MBAAm) as crosslinker directly in a plastic syringe for adsorption of albumin. The monolithic cryogel contained a continuous polymeric matrix having interconnected pores of 10–50 μm size. Poly(HEMA-co-DIPPER) cryogel was characterized by swelling studies, FTIR, scanning electron microscopy, and elemental analysis. The equilibrium swelling degree of the poly(HEMA-co-DIPPER) cryogel was 14.7 g H2O/g dry cryogel. Poly(HEMA-co-DIPPER) cryogel was used in the adsorption/desorption of albumin from aqueous solutions. The nonspecific adsorption of albumin onto plain poly(HEMA) cryogel was very low (3.36 g/g polymer). The maximum amount of albumin adsorption from aqueous solution in acetate buffer was 40.9 mg/g polymer at pH 5.0. It was observed that albumin could be repeatedly adsorbed and desorbed with the poly(HEMA-co-DIPPER) cryogel without significant loss of adsorption capacity.

Keywords

Cryogel Affinity separation Albumin HEMA 

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Nesibe Avcibaşi
    • 1
  • Murat Uygun
    • 2
  • M. Emin Çorman
    • 2
  • Sinan Akgöl
    • 3
    Email author
  • Adil Denizli
    • 4
  1. 1.Ege Vocational and Training SchoolEge UniversityIzmirTurkey
  2. 2.Department of Arts Chemistry, Faculty of ScienceAdnan Menderes UniversityAydinTurkey
  3. 3.Department of Biochemistry, Faculty of ScienceEge UniversityIzmirTurkey
  4. 4.Department of Chemistry, Faculty of ScienceHacettepe UniversityAnkaraTurkey

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