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Applied Biochemistry and Biotechnology

, Volume 162, Issue 8, pp 2197–2213 | Cite as

Catalytic Properties of Two Rhizopus oryzae 99-880 Glucoamylase Enzymes Without Starch Binding Domains Expressed in Pichia pastoris

  • Jeffrey A. MertensEmail author
  • Jay D. Braker
  • Douglas B. Jordan
Article

Abstract

Catalytic properties of two glucoamylases, AmyC and AmyD, without starch binding domains from Rhizopus oryzae strain 99-880 are determined using heterologously expressed enzyme purified to homogeneity. AmyC and AmyD demonstrate pH optima of 5.5 and 6.0, respectively, nearly one unit higher than the Rhizopus AmyA glucoamylase enzyme. Optimal initial activities are at 60 and 50 °C for AmyC and AmyD, respectively. Inactivation of both enzymes occurs at 50 °C following 30 min pre-incubation. The two enzymes demonstrate substantially slower catalytic rates toward soluble starch relative to AmyA. AmyC has similar k cat and K m for oligosaccharides to other Rhizopus and Aspergillus glucoamylases; however, the enzyme has a 2-fold lower K m maltose . AmyD has a 3-fold higher K m and lower k cat for maltooligosaccharides than AmyC and other glucoamylases. AmyC (but not AmyD) exhibits substrate inhibition. K i for substrate inhibition decreases with increasing length of the oligosaccharides. Data from pre-steady-state binding of AmyC to maltose and maltotriose and pre-steady-state to steady-state catalytic turnover experiments of AmyC acting on maltotriose were used to interrogate models of substrate inhibition. In the preferred model, AmyC accumulates an enzyme-maltose-maltotriose dead-end complex in the steady state.

Keywords

Glucoamylase Glycoside hydrolase Substrate inhibition Enzyme mechanism Pre-steady-state kinetics Rhizopus oryzae Pichia pastoris 

Notes

Acknowledgments

We thank Michael Bowman for MALDI-TOF analysis of the expressed AmyC and AmyD proteins.

Supplementary material

12010_2010_8994_MOESM1_ESM.doc (495 kb)
ESM 1 (DOC 495 kb)

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Copyright information

© US Government 2010

Authors and Affiliations

  • Jeffrey A. Mertens
    • 1
    Email author
  • Jay D. Braker
    • 1
  • Douglas B. Jordan
    • 1
  1. 1.Bioenergy Research Unit, National Center for Agricultural Utilization ResearchUSDA Agricultural Research ServicePeoriaUSA

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