Abstract
An effective protocol was developed for in vitro regeneration of the Cassia angustifolia via indirect organogenesis from petiole explants excised from 21-day-old axenic seedlings. Organogenic callus were induced on Murashige and Skoog (MS) medium supplemented with 5.0 µM 2,4-dichlorophenoxy acetic acid and 2.5 µM thidiazuron (TDZ). Adventitious shoot regeneration was achieved on MS medium supplemented with 5.0 µM TDZ as it induced 8.5 ± 0.98 shoots in 85% cultures. The number of shoots and shoot length was significantly enhanced when cultures were subcultured on auxin–cytokinin-containing medium. The highest number of shoots (12.5 ± 1.10) and shoot length (4.3 ± 0.20 cm) was recorded on MS medium supplemented with 5.0 µM TDZ and 1.5 µM indole-3-acetic acid. Regenerated shoots were rooted best on MS medium supplemented with 10.0 µM indole-3-butyric acid followed by their transfer to liquid MS filter paper bridge medium. The plants were successfully hardened off in sterile soilrite followed by their establishment in garden soil with 70% survival rate. The plants showed normal morphological characteristics similar to the field grown plants.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Anonymous. (1992). The wealth of India: A dictionary of Indian raw materials and industrial products (pp. 354–363). New Delhi: CSIR.
Franz, G. (1993). Pharmacology, 47, 2–6.
Agrawal, V., & Sardar, P. R. (2003). Physiology and Molecular Biology of Plants, 9, 131–140.
Siddique, I., & Anis, M. (2007). Acta Physiologiae Plantarum, 29, 233–238.
Siddique, I., & Anis, M. (2007). Medicinal and Aromatic Plant Science and Biotechnology, 1, 282–284.
Agrawal, V., & Sardar, P. R. (2006). Biologia Plantarum, 50, 118–122.
Agrawal, V., & Sardar, P. R. (2007). In Vitro Cellular and Developmental Biology of Plants, 43, 585–592.
Murashige, T., & Skoog, F. (1962). Physiologia Plantarum, 15, 473–497.
Faisal, M., Ahmad, N., & Anis, M. (2006). Phytomorphology, 56, 183–187.
Malik, K. A., & Saxena, P. K. (1992). Planta, 186, 384–389.
Huetteman, C. A., & Preece, J. E. (1993). Plant Cell Tissue and Organ Culture, 33, 105–119.
Tiwari, V., Tiwari, K. N., & Singh, B. D. (2001). Plant Cell Tissue and Organ Culture, 66, 9–16.
Faisal, M., Singh, S., & Anis, M. (2005). In Vitro Cellular and Developmental Biology of Plants, 41, 511–515.
Baskaran, P., Velayutham, P., & Jayabalan, N. (2009). In Vitro Cellular and Developmental Biology of Plants, 45, 407–413.
Shailendra, V., Joshi, N., Tak, K., & Purohit, S. D. (2005). In Vitro Cellular and Developmental Biology of Plants, 41, 296–302.
Faisal, M., & Anis, M. (2003). Plant Cell Tissue and Organ Culture, 75, 125–129.
Faisal, M., Siddique, I., & Anis, M. (2006). In Vitro Cellular and Developmental Biology of Plants, 42, 59–64.
Siddique, I., & Anis, M. (2008). Acta Physiologiae Plantarum, 30, 493–499.
Martin, K. P. (2002). Plant Cell Reports, 21, 112–117.
Acknowledgments
The award of a Research Associateship to I. S. by the Council of Scientific and Industrial Research, Government of India, New Delhi, is gratefully acknowledged. Authors are also grateful to the Department of Science and Technology, and the University Grants Commission Government of India, New Delhi for providing research support under DST-FIST (2005) and UGC-SAP (2009) Programs respectively.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Siddique, I., Anis, M. & Aref, I.M. In Vitro Adventitious Shoot Regeneration via Indirect Organogenesis from Petiole Explants of Cassia angustifolia Vahl.—a Potential Medicinal Plant. Appl Biochem Biotechnol 162, 2067–2074 (2010). https://doi.org/10.1007/s12010-010-8982-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12010-010-8982-4