Skip to main content
SpringerLink
Log in

Study of Starch Degradation by Yeasts During Fermentation for Using in Animal Feed

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

Sixteen amylolytic yeasts, drawn from culture collections or isolated from different environments, were used in the present study. Experiments were performed to evaluate the starch degradation due to exocellular activity. The culture media of growth was optimized to find the maximum activity, and some strains produced an extracellular amylolytic enzyme when cultured in presence of starch in the medium. On the other hand, one yeast showed activity in cell extract when cultured under varying conditions in a bench bioreactor; this strain would thus be suitable for use as an animal feed supplement, both as a source of proteins and nucleic acids and in terms of its amylolytic activity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Beauchemin, K. A., & Eun, J. S. (2007). Enzymes to improve forage utilization by ruminants: What's on the horizon. Journal of Animal Science, 85, 237–237.

    Article  Google Scholar 

  2. Jouany, J. P., & Morgavi, D. R. (2007). Use of ‘natural’ products as alternatives to antibiotic feed additives in ruminant production. Animal, 1, 1443–1466.

    Article  CAS  Google Scholar 

  3. Eun, J. S., Beauchemin, K. A., & Schulze, H. (2007). Use of exogenous fibrolytic enzymes to enhance in vitro fermentation of alfalfa hay and corn silage. Journal of Dairy Science, 3, 1440–1451.

    Article  Google Scholar 

  4. Graminha, E. B. N., Gongalves, A. Z. L., Pirota, R. D. P. B., Balsalobre, M. A. A., Da Silva, R., & Gomes, E. (2008). Enzyme production by solid-state fermentation: Application to animal nutrition. Animal Feed Science and Technology, 144, 1–22.

    Article  CAS  Google Scholar 

  5. Varel, V. H., & Kreikemeier, K. K. (1994). Influence of feeding Aspergillus-oryzae fermentation extract (amaferm) on in-situ fiber degradation, ruminal fermentation, and microbial protein-synthesis in nonlactating cows fed alfalfa or bromegrass hay. Journal of Animal Science, 72, 1814–1822.

    CAS  Google Scholar 

  6. Carreiro, S. C., Pagnocca, F. C., & Bacci, M. (2004). Sympodiomyces attinorum sp nov., a yeast species associated with nests of the leaf-cutting ant Atta sexdens. International Journal of Systematic and Evolutionary Microbiology, 54, 1891–1894.

    Article  CAS  Google Scholar 

  7. Takeuchi, A., Shimizu-Ibuka, A., & Nishiyama, Y. (2006). Purification and characterization of an alpha-amylase of Pichia burtonii isolated from the traditional starter “murcha” in Nepal. Bioscience, Biotechnology, and Biochemistry, 70, 3019–3024.

    Article  CAS  Google Scholar 

  8. Shewale, S., & Pandit, A. B. (2007). Hydrolisis of soluble starch using Bacillus licheniformis α-amylase inmobilized on superporous CELBEADS. Carbohydrate Research, 343, 997–1008.

    Article  Google Scholar 

  9. Xu, H., Sun, L. P., & Zhao, D. Q. (2008). Production of alpha-amylase by Aspergillus oryzae As 3951 in solid state fermentation using spent brewing grains as substrate. Journal of the Science of Food and Agriculture, 88, 529–535.

    Article  CAS  Google Scholar 

  10. DeFrain, J. M., Hippen, A. R., Kalscheur, K. F., & Tricarico, J. M. (2005). Effects of dietary alpha-amylase on metabolism and performance of transition dairy cows. Journal of Dairy Science, 88, 4405–4413.

    Article  CAS  Google Scholar 

  11. Tricarico, J. M., Johnston, J. D., & Dawson, K. A. (2008). Dietary supplementation of ruminant diets with an Aspergillus oryzae alpha-amylase. Animal Feed Science and Technology, 145, 136–150.

    Article  CAS  Google Scholar 

  12. Rojo, R., Mendoza, G. D., Gonzalez, S. S., Landois, L., Barcena, R., & Crosby, M. M. (2005). Effects of exogenous amylases from Bacillus licheniformis and Aspergillus niger on ruminal starch digestion and lamb performance. Animal Feed Science and Technology, 124, 655–665.

    Article  Google Scholar 

  13. Gutiérrez, C. L. C., Mendoza, M. G. D., Pinos, R. J. M., Ricalde, R., Aranda, E., & Miranda, L. A. (2005). Effects of storage time and processing temperature of grains with added amylolytic enzymes on in situ ruminal starch digestion. Journal of Applied Animal Research, 27, 39–44.

    Google Scholar 

  14. Rose, M.D., Winston, F., Hieter, P. (1990). Methods in yeast genetics: a laboratory course manual. In: Methods in yeast genetics: a laboratory course manual (pp 131–132). New York: Cold Spring Harbour Laboratory Press.

Download references

Author information

Authors and Affiliations

  1. Regional Institute of Applied Scientific Research (RIASR), Universidad de Castilla-La Mancha, Campus Universitario, 13071, Ciudad Real, Spain

    Santiago Alonso, Maria Arévalo-Villena, Juan Úbeda & Ana Briones

Authors
  1. Santiago Alonso
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Maria Arévalo-Villena
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Juan Úbeda
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ana Briones
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Maria Arévalo-Villena.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Alonso, S., Arévalo-Villena, M., Úbeda, J. et al. Study of Starch Degradation by Yeasts During Fermentation for Using in Animal Feed. Appl Biochem Biotechnol 162, 2058–2066 (2010). https://doi.org/10.1007/s12010-010-8981-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-010-8981-5

Keywords

Search

Navigation