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Applied Biochemistry and Biotechnology

, Volume 162, Issue 7, pp 2027–2036 | Cite as

High-Level Expression and Efficient Purification of Bioactive Swollenin in Aspergillus oryzae

  • Meihua Wang
  • Jin Cai
  • Lei HuangEmail author
  • Zhengbin Lv
  • Yaozhou Zhang
  • Zhinan XuEmail author
Article

Abstract

The bioactivity of swollenin is beneficial to cellulose decomposition by cellulase despite the lack of hydrolytic activity itself. In order to improve the productivity of swollenin, the effects of culture conditions on the expression level in recombinant Aspergillus oryzae were investigated systematically. With regard to the bioactivity of swollenin, glycerin and peanut meal were the optimal carbon or nitrogen source, respectively. The highest level production of swollenin (50 mg L−1) was attained after 88 h cultivation with the initial pH of 5.6 in the culture medium. Then the soluble swollenin was effectively purified from the cultural supernatant by ammonium sulfate precipitation and cationic exchange chromatography with recovery yield of 53.2%. The purified swollenin was fully bioactive due to its strong synergistic activity with cellulose.

Keywords

Aspergillus oryzae Swollenin Purification Soluble expression Synergistic activity 

Notes

Acknowledgments

This work was financially supported by the National High Technology Research and Development Program of China (2009AA02Z206), the National Basic Research Program of China (2007CB707805), and Nature Science Foundation of China (20736008, 20676115).

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Institute of Bioengineering, Department of Chemical and Biological EngineeringZhejiang UniversityHangzhouChina
  2. 2.Institute of BiochemistryZhejiang Sci-Tech UniversityHangzhouChina

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