Advertisement

Applied Biochemistry and Biotechnology

, Volume 162, Issue 7, pp 1915–1928 | Cite as

Integration of Succinic Acid and Ethanol Production With Potential Application in a Corn or Barley Biorefinery

  • Nhuan P. NghiemEmail author
  • Kevin B. Hicks
  • David B. Johnston
Article

Abstract

Production of succinic acid from glucose by Escherichia coli strain AFP184 was studied in a batch fermentor. The bases used for pH control included NaOH, KOH, NH4OH, and Na2CO3. The yield of succinic acid without and with carbon dioxide supplied by an adjacent ethanol fermentor using either corn or barley as feedstock was examined. The carbon dioxide gas from the ethanol fermentor was sparged directly into the liquid media in the succinic acid fermentor without any pretreatment. Without the CO2 supplement, the highest succinic acid yield was observed with Na2CO3, followed by NH4OH, and lowest with the other two bases. When the CO2 produced in the ethanol fermentation was sparged into the media in the succinic acid fermentor, no improvement of succinic acid yield was observed with Na2CO3. However, several-fold increases in succinic acid yield were observed with the other bases, with NH4OH giving the highest yield increase. The yield of succinic acid with CO2 supplement from the ethanol fermentor when NH4OH was used for pH control was equal to that obtained when Na2CO3 was used, with or without CO2 supplementation. The benefit of sparging CO2 from ethanol fermentation on the yield of succinic acid demonstrated the feasibility of integration of succinic acid fermentation with ethanol fermentation in a biorefinery for production of fuels and industrial chemicals.

Keywords

Succinic acid Escherichia coli AFP184 CO2 fixation Ethanol fermentation Biorefinery 

Notes

Acknowledgement

The authors would like to thank Gerard Senske and John Minutolo for skillfully performing the fermentation experiments and Michael Kurantz for the compositional analysis of the barley and corn used in this study. The many invaluable comments and suggestions of Dr. Susanne Kleff of MBI International, Lansing, MI, given during the review of the first draft of the manuscript also are greatly appreciated.

References

  1. 1.
    Werpy, T., & Petersen, G. (2004). Top value added chemicals from biomass, U.S. Department of Energy.Google Scholar
  2. 2.
    Fumagalli, C. (1997). In J. I. Kroschwitz & M. Howe-Grant (Eds.), Kirk-Othmer encyclopedia of chemical technology Vol. 22 (pp. 1072–1102). New York, NY: Wiley.Google Scholar
  3. 3.
    Rao, V. N. M. (1988). U.S. Patent 4,782,167.Google Scholar
  4. 4.
    Mabry, M. A. (1985). U.S. Patent 4,550,185.Google Scholar
  5. 5.
    Berglund, K. A., Dunuwila, D. D., Alizadeh, H. (2003). U. S. Patent 6,623,657.Google Scholar
  6. 6.
    Berglund, K. A., Dunuwila, D. D., Alizadeh, H. (2003). U.S. Patent 6,635,188.Google Scholar
  7. 7.
    Glassner, D. A., & Datta, R. (1992). U.S. Patent 5,143,834.Google Scholar
  8. 8.
    Guettler, M. V., Jain, M. K., Soni, B. K. (1996). U.S. Patent 5,504,004.Google Scholar
  9. 9.
    Nghiem, N. P., Donnelly, M., Millard, C. S. (1999). U.S. Patent 5,869,301.Google Scholar
  10. 10.
    Donnelly, M., & Nghiem, N. P. (2004). U.S. Patent 6,743,610.Google Scholar
  11. 11.
    Lin, H., Bennett, G. N., & San, K. Y. (2005). Biotechnology and Bioengineering, 90, 775–779.CrossRefGoogle Scholar
  12. 12.
    Lee, S. J., Song, H., & Lee, S. Y. (2006). Applied and Environmental Microbiology, 72, 1939–1948.CrossRefGoogle Scholar
  13. 13.
    Okino, S., Inui, M., & Yukawa, H. (2005). Applied Microbiology and Biotechnology, 68, 475–480.CrossRefGoogle Scholar
  14. 14.
    Nghiem, N. P., Davison, B. H., Donnelly, M. I., Tsai, S. -P., Frye, J. G. (2000). Chemicals and materials from renewable resources. In J. J. Bozell (Ed.), American chemical society symposium series 784:160–173.Google Scholar
  15. 15.
    Millard, C. S., Chao, Y.-P., Liao, J. C., & Donnelly, M. I. (1996). Appl Environmental Microbiology, 62, 1808–1810.Google Scholar
  16. 16.
    Vemuri, G. N., Eiteman, M. A., & Altman, E. (2002). Journal of Industrial Microbiology & Biotechnology, 28, 325–332.CrossRefGoogle Scholar
  17. 17.
    Anderson, C., Hodge, D., Berglund, K. A., & Rova, U. (2007). Biotechnology Progress, 23, 381–388.CrossRefGoogle Scholar
  18. 18.
    Anderson, C., Helmerius, J., Hodge, D., Berglund, K. A., & Rova, U. (2009). Biotechnology Progress, 25, 116–123.CrossRefGoogle Scholar
  19. 19.
    Drapcho, C. M., Nghiem, N. P., & Walker, T. H. (2008). Biofuels engineering process technology Chapter 5 (pp. 105–195). New York: McGraw-Hill.Google Scholar
  20. 20.
    Schill, S. R. (2008). Ethanol producer magazine, October Issue.Google Scholar
  21. 21.
    Gottschalk, G. (1986). Bacterial metabolism, Chapter 5 (2nd ed., pp. 127–129). New York: Springer-Verlag.Google Scholar
  22. 22.
    Zeikus, J. G., Jain, M. K., & Elankovan, P. (1999). Applied and Environmental Microbiology, 51, 545–552.Google Scholar
  23. 23.
    McKinlay, J. B., Vieille, C., & Zeikus, J. G. (2007). Applied and Environmental Microbiology, 76, 727–740.Google Scholar
  24. 24.
    Lu, S., Eiteman, M. A., & Altman, E. (2009). Journal of Industrial Microbiology & Biotechnology, 36, 1101–1109.CrossRefGoogle Scholar

Copyright information

© US Government 2010

Authors and Affiliations

  • Nhuan P. Nghiem
    • 1
    Email author
  • Kevin B. Hicks
    • 1
  • David B. Johnston
    • 1
  1. 1.U.S. Department of AgricultureEastern Regional Research Center, Agricultural Research ServiceWyndmoorUSA

Personalised recommendations