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Applied Biochemistry and Biotechnology

, Volume 162, Issue 7, pp 1901–1914 | Cite as

Bacterially Expressed Double-Stranded RNAs against Hot-Spot Sequences of Tobacco Mosaic Virus or Potato Virus Y Genome Have Different Ability to Protect Tobacco from Viral Infection

  • Zhao-Nan Sun
  • Guo-Hua Yin
  • Yun-Zhi Song
  • Hai-Long An
  • Chang-Xiang ZhuEmail author
  • Fu-Jiang WenEmail author
Article

Abstract

Posttranscriptional gene silencing, also known as RNA interference, involves degradation of homologous mRNA sequences in organisms. In plants, posttranscriptional gene silencing is part of a defense mechanism against virus infection, and double-stranded RNA is the pivotal factor that induces gene silencing. In this paper, we got seven hairpin RNAs (hpRNAs) constructs against different hot-spot sequences of Tobacco mosaic virus (TMV) or Potato virus Y (PVY) genome. After expression in Escherichia coli HT115, we extracted the seven hpRNAs for the test in tobacco against TMV or PVY infection. The data suggest that different hpRNAs against different hot-spot sequences of TMV or PVY genome had different ability to protect tobacco plants from viral infection. The resistance to TMV conferred by the hpRNA against the TMV movement protein was stronger than other TMV hpRNAs; the resistance to PVY conferred by the hpRNA against the PVY nuclear inclusion b was better than that induced by any other PVY hpRNAs. Northern blotting of siRNA showed that the resistance was indeed an RNA-mediated virus resistance.

Keywords

Tobacco mosaic virus Potato virus Y RNA interference hpRNAs Hot-spot sequences 

Notes

Acknowledgement

We acknowledge Li Wei for the kind gift of the bacterial strain HT115. This work was supported by the National Natural Science Foundation of China (No. 30771408) and the Excellent Youth and Middle Age Scientists Fund of Shan Dong Province (No. 2007BS06007).

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.College of Life Sciences, State Key Laboratory of Crop BiologyShandong Agricultural UniversityTai’anChina

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