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Isolation and Identification of Two New Fungal Strains for Xylanase Production

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Abstract

Fungi are well known for their ability to excrete enzymes into the environment. The aim of this work was to evaluate xylanase production by fungi isolated from soil. One hundred and thirty-six fungal isolates were screened for xylanase production. Two xylanase producing isolates, FSS117 and FSS129, were identified on the basis of analyses of 5,8S gene sequencing. The closest phylogenetic neighbors according to 5,8S gene sequence data for the two isolates were Aspergillus tubingensis and Aspergillus terreus, respectively. When birchwood xylan or corn cob hulls was used as a substrate for 5 days under submerged culture cultivation, xylanase production from A. terreus FSS129 was 113 and 174 IU ml−1, respectively. The pH and temperature for optimum xylanase activity were 8 and 65 ºC.

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Acknowledgment

We are grateful to the Atomic Energy Commission of Syria (AECS) who financially supported this research which was realized in both the Centre Wallon de Biologie Industrielle (CWBI), Gembloux Agricultural University, Belgium and the Atomic Energy Commission of Syria (AECS), Department of Molecular Biology and Biotechnology, Damascus, Syria.

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Authors and Affiliations

  1. Department of Molecular Biology and Biotechnology, AECS, P. O. Box 6091, Damascus, Syria

    Yasser Bakri

  2. Unité de Bio-industries, Faculté Universitaire des Sciences Agronomiques de Gembloux, Centre Wallon de Biologie Industrielle, Passage des Déportes 2, 5030, Gembloux, Belgium

    Magali Masson & Philippe Thonart

Authors
  1. Yasser Bakri
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  2. Magali Masson
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  3. Philippe Thonart
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Correspondence to Yasser Bakri.

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Bakri, Y., Masson, M. & Thonart, P. Isolation and Identification of Two New Fungal Strains for Xylanase Production. Appl Biochem Biotechnol 162, 1626–1634 (2010). https://doi.org/10.1007/s12010-010-8944-x

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  • DOI: https://doi.org/10.1007/s12010-010-8944-x

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