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Cytokines Expression Induced by Ganoderma sinensis Fungal Immunomodulatory Proteins (FIP-gsi) in Mouse Spleen Cells

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Abstract

Ganoderma sinensis fungal immunomodulatory protein (FIP-gsi) was a new member of FIPs family. Based on the cloning of FIP-gsi gene from G. sinensis, this paper reported that FIP-gsi gene was expressed in Escherichia coli expression system. Then, the recombinant proteins were analyzed by the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Finally, the immunomodulatory activity was examined by inducing cytokine genes expression. The results showed that the recombinant FIP-gsi protein could be expressed in E. coli and got the yield of about 25% of the soluble form in the total soluble protein. The FIP-gsi protein was composed of 111 amino acids, and the sequence of homologous rate was 88.6% with FIP-glu (LZ-8). Furthermore, it could enhance the levels of interleukin (IL)-2, IL-3, IL-4, interferon gamma, tumor necrosis factor alpha, and IL-2 receptor (IL-2R) in mouse spleen cells.

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References

  1. Zhou, X. W., Lin, J., Yin, Y. Z., Zhao, J. Y., Sun, X. F., & Tang, K. X. (2007). American Journal of Chinese Medicine, 35, 559–574.

    Article  CAS  Google Scholar 

  2. Chinese Pharmacopoeia Commission. (2005). Pharmacopoeia of the People's Republic of China, 2005 ed, Beijing.

  3. Zjawiony, J. K. (2004). Journal of Natural Products, 67, 300–310.

    Article  CAS  Google Scholar 

  4. Zhou, X. W., Lin, J., Li, Q. Z., Yin, Y. Z., Sun, X. F., & Tang, K. X. (2007). Natural Product Research and Development, 19, 917–924.

    CAS  Google Scholar 

  5. Kino, K., Yamashita, A., Yamaoka, K., Watanabe, J., Tanaka, S., Ko, K., et al. (1989). Journal of Biological Chemistry, 264, 472–478.

    CAS  Google Scholar 

  6. Ngai, P. H., & Ng, T. B. (2004). Biochemical and Biophysical Research Communications, 314, 988–993.

    Article  CAS  Google Scholar 

  7. Lin, W. H., Hung, C. H., Hsu, C. I., & Lin, J. Y. (1997). Journal of Biological Chemistry, 272, 20044–20048.

    Article  CAS  Google Scholar 

  8. Ko, J. L., Hsu, C. I., Lin, R. H., Kao, C. L., & Lin, J. Y. (1995). European Journal of Biochemistry, 228, 244–249.

    Article  CAS  Google Scholar 

  9. Hsu, H. C., Hsu, C. I., Lin, R. H., Kao, C. L., & Lin, J. Y. (1997). Biochemical Journal, 323, 557–565.

    CAS  Google Scholar 

  10. Tanaka, S., Ko, K., Kino, K., Tsuchiya, K., Yamashita, A., Murasugi, A., et al. (1989). Journal of Biological Chemistry, 264, 16372–16377.

    CAS  Google Scholar 

  11. Paaventhan, P., Joseph, J. S., Seow, S. V., Vaday, S., Robinson, H., Chua, K. Y., et al. (2003). Journal of Molecular Biology, 332, 461–470.

    Article  CAS  Google Scholar 

  12. Haak-Frendscho, M., Kino, K., Sone, T., & Jardieu, P. (1993). Cellular Immunology, 150, 101–103.

    Article  Google Scholar 

  13. Yeh, C. M., Yeh, C. K., Hsu, X. Y., Luo, Q. M., & Lin, M. Y. (2008). Applied and Environmental Microbiology, 74, 1039–1049.

    Article  CAS  Google Scholar 

  14. Jinn, T. R., Wu, C. M., Tu, W. C., Ko, J. L., & Tzen, J. T. (2006). Bioscience Biotechnology and Biochemistry, 70, 2627–2634.

    Article  CAS  Google Scholar 

  15. Zhou, X. W., Xie, M. Q., Hong, F., Li, Q. Z., & Lin, J. (2009). International Journal of Medicinal Mushrooms, 11, 77–86.

    Article  CAS  Google Scholar 

  16. Lomedico, P. T., Gubler, U., Hellmann, C. P., Dukovich, M., Giri, J. G., Pan, Y. C., et al. (1984). Nature, 312, 458–462.

    Article  CAS  Google Scholar 

  17. Kashima, N., Nishi-Takaoka, C., Fujita, T., Taki, S., Yamada, G., Hamuro, J., et al. (1985). Nature, 313, 402–404.

    Article  CAS  Google Scholar 

  18. Fung, M. C., Hapel, A. J., Ymer, S., Cohen, D. R., Johnson, R. M., Campbell, H. D., et al. (1984). Nature, 307, 233–237.

    Article  CAS  Google Scholar 

  19. Otsuka, T., Villaret, D., Yokota, T., Takebe, Y., Lee, F., Arai, N., et al. (1987). Nucleic Acids Research, 15, 333–344.

    Article  CAS  Google Scholar 

  20. Kinashi, T., Harada, N., Severinson, E., Tanabe, T., Sideras, P., Konishi, M., et al. (1986). Nature, 324, 70–73.

    Article  CAS  Google Scholar 

  21. Van Snick, J., Cayphas, S., Szikora, J. P., Renauld, J. C., Van Roost, E., Boon, T., et al. (1988). European Journal of Immunology, 18, 193–197.

    Article  Google Scholar 

  22. Gray, P. W., & Goeddel, D. V. (1983). Proceeding of the National Academy Sciences of the United Sates of America, 80, 5842–5846.

    Article  CAS  Google Scholar 

  23. Pennica, D., Hayflick, J. S., Bringman, T. S., Palladino, M. A., & Goeddel, D. V. (1985). Proceeding of the National Academy Sciences of the United Sates of America, 82, 6060–6064.

    Article  CAS  Google Scholar 

  24. Turetskaya, R., Fashena, S. J., Paul, N. L., & Ruddle, N. H. (1992). In B. Aggarwal & J. Vilcek (Eds.), Tumor necrosis: structure, function and mechanism of action (pp. 35–60). New York: Marcel Dekker.

    Google Scholar 

  25. Miller, J., Malek, T. R., Leonard, W. J., Greene, W. C., Shevach, E. M., & Germain, R. N. (1985). Journal of Immunology, 134, 4212–4217.

    CAS  Google Scholar 

  26. Alonso, S., Minty, A., Bourlet, Y., & Buckingham, M. (1986). Journal of Molecular Evolution, 23, 11–22.

    Article  CAS  Google Scholar 

  27. Paterson, R. R. (2006). Phytochemistry, 67, 1985–2001.

    Article  CAS  Google Scholar 

  28. Chen, T. Q., Wu, J. Z., Zhong, L. Y., Li, Y., Qiu, F. P., Lin, X. S., et al. (2006). Journal of Fujian Agriculture and Forestry University (Natural Science Edition), 35, 324–328.

    CAS  Google Scholar 

  29. Chen, T. Q., Wu, J. Z., Li, Y., Huang, Q. D., Yu, D. Y., Lin, X. S., et al. (2006). Journal of Fungal Research, 4, 27–32.

    CAS  Google Scholar 

  30. Liu, C., Wang, H. Q., Li, B. M., & Chen, R. Y. (2007). China Journal of Chinese Materia Medica, 32, 235–237.

    CAS  Google Scholar 

  31. Bai, J. Y., Zeng, L., Li, Y. F., & Lin, Z. P. (2006). Practice Journal of Medicine and Pharmacology, 23, 1205–1207.

    Google Scholar 

  32. Huang, J., Zhang, L. X., Hu, S. N., & Yu, J. (2008). Journal of Zhejiang University (Agriculture and Life Science), 34, 7–12.

    CAS  Google Scholar 

  33. Ko, J. L., Lin, S. J., Hsu, C. I., Kao, C. L., & Lin, J. Y. (1997). Journal of the Formosan Medical Association, 96, 517–524.

    CAS  Google Scholar 

  34. Wang, H. M., Zhang, P., Huang, L. J., & Wang, Z. F. (2009). Progress in Chemistry, 21, 1335–1343.

    Google Scholar 

  35. Lewis, J. K., Wei, J., & Siuzdak, G. (2000). In R. A. Meyers (Ed.), Matrix-assisted laser desorption/ionization mass spectrometry in peptide and protein analysis, encyclopedia of analytical chemistry (pp. 5880–5994). Chichester: Wiley & Sons, J. Ltd.

    Google Scholar 

  36. Dalluge, J. J. (2002). Analytical and Bioanalytical Chemistry, 372, 18–19.

    Article  CAS  Google Scholar 

  37. Maggi, E., Biswas, P., Del Prete, G., Parronchi, P., Macchia, D., Simonelli, C., et al. (1991). Journal of Immunology, 146, 1169–1174.

    CAS  Google Scholar 

  38. Romagnani, S. (1991). Immunology Today, 12, 256–257.

    Article  CAS  Google Scholar 

  39. Rengarajan, J., Szabo, S. J., & Glimcher, L. H. (2000). Immunology Today, 21, 479–483.

    Article  CAS  Google Scholar 

  40. Hu, J. Z., & Li, Y. (2009). West China Medical Journal, 24, 236–237.

    CAS  Google Scholar 

  41. Wang, Y. H., Xu, B., & Xi, X. X. (2008). Journal of Shaanxi College of Traditional Chinese Medicine, 31, 5–6.

    CAS  Google Scholar 

  42. Nakamura, T., Lee, R. K., Nam, S. Y., Podack, E. R., Bottomly, K., & Flavell, R. A. (1997). Journal of immunology, 158, 2648–2653.

    CAS  Google Scholar 

Download references

Acknowledgements

This research is financially supported by the National Natural Science Foundation of China under Grant Nos. 30771500, Shanghai Science and Technology Committee under Grant Nos.05DZ05007 and Shanghai Leading Academic Discipline Project (Project Number: B209).

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Authors and Affiliations

  1. Plant Biotechnology Research Center, Shanghai Key Laboratory of Agrobiotechnology, School of Agriculture and Biology, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Shanghai Jiao Tong University, Shanghai, 200240, People’s Republic of China

    Qizhang Li, Xuefei Wang & Xuanwei Zhou

  2. State of Key Laboratory of Genetic Engineering, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Life Science, Fudan University, Shanghai, 200433, China

    Yiyuan Chen & Juan Lin

  3. Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, People’s Republic of China

    Juan Lin & Xuanwei Zhou

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  1. Qizhang Li
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  2. Xuefei Wang
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  4. Juan Lin
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Correspondence to Xuanwei Zhou.

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Li, Q., Wang, X., Chen, Y. et al. Cytokines Expression Induced by Ganoderma sinensis Fungal Immunomodulatory Proteins (FIP-gsi) in Mouse Spleen Cells. Appl Biochem Biotechnol 162, 1403–1413 (2010). https://doi.org/10.1007/s12010-010-8916-1

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