Abstract
Lactobacillus bulgaricus grown on whey was dried by a simple thermal drying method at the range 35–55°C and its efficiency for lactic acid fermentation of whey was evaluated. Drying of cells in whey suspension in the examined temperature range did not affect significantly their viability (82–87% survival), indicating a protective effect of whey as both growth and drying medium. The kinetics of fermentation of whey and mixtures of whey/molasses using the dried culture were comparable to those of non-dried cells, and only low pH had a detrimental effect on the fermentation ability of the dried cells. Furthermore, dried L. bulgaricus, free or immobilized on casein coagulates, was used as starter for the production of unsalted hard-type cheese. The effects of the amount of starter culture and the immobilization technique, the evolution of microbial counts, and the sensory properties of the produced cheeses were evaluated during ripening at various temperatures.
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References
Steinkraus, K. H. (1994). Food Research International, 27, 259–267.
Ammor, M. S., & Mayo, B. (2007). Meat Science, 76, 138–146.
De Vuyst, L., & Neysens, P. (2005). Trends in Food Science & Technology, 16, 43–56.
Ross, R. P., Stanton, C., Hill, C., Fitzgerald, G. F., & Coffey, A. (2000). Trends in Food Science & Technology, 11, 96–104.
Santivarangkna, C., Kulozik, U., & Foerst, P. (2007). Biotechnology Progress, 23, 302–315.
Morgan, C. A., Herman, N., White, P. A., & Vesey, G. (2006). Journal of Microbiological Methods, 66, 183–193.
Linders, L. J. M., Kets, E. P. W., de Bont, J. A. M., & Van’t Riet, K. (1998). Biotechnology Progress, 14, 537–539.
Carvalho, A. S., Silva, J., Ho, P., Teixeira, P., Malcata, F. X., & Gibbs, P. (2004). Biotechnology Progress, 20, 248–254.
Shene, C., & Bravo, S. (2007). Enzyme and Microbial Technology, 40, 1578–1584.
Tymczyszyn, E. E., Gomez-Zavaglia, A., & Disalvo, E. A. (2007). Journal of Applied Microbiology, 102, 845–851.
Huang, L., Lu, Z., Yuan, Y., Lü, F., & Bie, X. (2006). Journal of Industrial Microbiology & Biotechnology, 33, 55–61.
Silva, J., Carvalho, A. S., Ferreira, R., Vitorino, R., Amado, F., Domingues, P., et al. (2005). Journal of Applied Microbiology, 98, 775–782.
Plessas, S., Pherson, L., Bekatorou, A., Nigam, P., & Koutinas, A. A. (2005). Food Chemistry, 93, 585–589.
Plessas, S., Trantallidi, M., Bekatorou, A., Kanellaki, A., Nigam, P., & Koutinas, A. A. (2007). Food Chemistry, 105, 187–194.
Kourkoutas, Y., Kandylis, P., Panas, P., Dooley, J. S. G., Nigam, P., & Koutinas, A. A. (2006). Applied and Environmental Microbiology, 72, 6124–6135.
Katechaki, E., Panas, P., Kandilogiannakis, L., Rapti, K., & Koutinas, A. A. (2008). Journal of Agricultural and Food Chemistry, 56, 5316–5323.
Dimitrellou, D., Kourkoutas, Y., Banat, I. M., Marchant, R., & Koutinas, A. A. (2007). Journal of Applied Microbiology, 103, 1170–1183.
Koutinas, A. A., Athanasiadis, I., Bekatorou, A., Iconomopoulou, M., & Blekas, G. (2005). Biotechnology and Bioengineering, 89, 788–796.
Giorno, L., Chojnacka, K., Donato, L., & Drioli, E. (2002). Industrial & Engineering Chemistry Research, 41, 433–440.
Gandhi, D. N., Patel, R. S., Wadhwa, B. K., Neena, B., Manjeet, K., & Ganesh-Kumar, C. (2000). J Food Sci Tech Mys, 37, 292–295.
Ashar, M. N., & Chand, R. (2004). Milchwissenschaft, 59, 14–17.
Bury, D., Hajsmanova, M., & Jelen, P. (1999). Milchwissenschaft, 54, 610–612.
AOAC. (1995). Chapter 33. Dairy products. Method 947.05. In: AOAC (Eds.) Acidity in milk. Official methods of analysis of AOAC International, 16th Edition, vol. II.
Koutinas, A. A., Papapostolou, H., Dimitrellou, D., Kopsahelis, N., Katechaki, E., Bekatorou, A., et al. (2009). Biores Technol, 100, 3734–3739.
Tsaousi, K., Koutinas, A. A., Bekatorou, A., & Loukatos, P. (2009). Applied biochemistry and biotechnology, In press. doi:10.1007/s12010-009-8848-9.
Dimitrellou, D., Kandylis, P., & Kourkoutas, Y. (2009). Koutinas, Kanellaki, M. Food Chemistry, 115, 691–696.
Papapostolou, H., Bosnea, L. A., Koutinas, A. A., & Kanellaki, M. (2008). Biores Technol, 99, 6949–6956.
Bucio, A., Hartemink, R., Schrama, J. W., & Rombouts, F. (2005). Journal of General and Applied Microbiology, 51, 221–227.
Dimitrellou, D., Kourkoutas, Y., Koutinas, A. A., & Kanellaki, M. (2009). Food Microbiology, 26, 809–820.
Katechaki, E., Panas, P., Kourkoutas, Y., Koliopoulos, D., & Koutinas, A. A. (2009). Biores Technol, 100, 3618–3624.
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This work was performed within the framework of the Regional Operational Programme (ROP) of Western Greece and was cofunded by the European Regional Development Fund and the Region of Western Greece with final beneficiary the Greek General Secretariat for Research and Technology.
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Katechaki, E., Solomonidis, T., Bekatorou, A. et al. Thermal Drying of Lactobacillus delbrueckii subsp. bulgaricus and its Efficient Use as Starter for Whey Fermentation and Unsalted Cheese Making. Appl Biochem Biotechnol 162, 1270–1285 (2010). https://doi.org/10.1007/s12010-009-8904-5
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DOI: https://doi.org/10.1007/s12010-009-8904-5