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Applied Biochemistry and Biotechnology

, Volume 162, Issue 3, pp 719–732 | Cite as

Isolation and Screening of Microorganisms for R-(+)-Limonene and (−)-β-Pinene Biotransformation

  • Ieda Rottava
  • Priscila F. Cortina
  • Camila E. Grando
  • André R. S. Colla
  • Eduarda Martello
  • Rogério L. Cansian
  • Geciane Toniazzo
  • Helen TreichelEmail author
  • Octávio A. C. Antunes
  • Enrique G. Oestreicher
  • Débora de Oliveira
Article

Abstract

This work is focused on the biotransformation of R-(+)-limonene and (−)-β-pinene to bioflavor production. To carry out the present study, 405 microorganisms were tested for their ability to bioconvert the substrates. From the isolated microorganisms, 193 were selected in the prescreening using mineral medium for limonene degradation. At the screening step, eight strains were able to convert R-(+)-limonene and 15 to transform (−)-β-pinene, both in α-terpineol. The highest concentration in α-terpineol from R-(+)-limonene was about 3,450 mg/L for Penicillium sp. isolated from eucalyptus steam. From (−)-β-pinene, the highest product concentration of 675.5 mg/L was achieved using an Aspergillus sp. strain isolated from orange tree stem.

Keywords

Screening Biotransformation R-(+)-limonene (−)-β-Pinene α-Terpineol Monoterpenes 

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Ieda Rottava
    • 1
  • Priscila F. Cortina
    • 2
  • Camila E. Grando
    • 2
  • André R. S. Colla
    • 2
  • Eduarda Martello
    • 2
  • Rogério L. Cansian
    • 2
  • Geciane Toniazzo
    • 2
  • Helen Treichel
    • 2
    Email author
  • Octávio A. C. Antunes
    • 1
  • Enrique G. Oestreicher
    • 1
  • Débora de Oliveira
    • 2
  1. 1.Department of BiochemistryInstituto de Química–UFRJ, CTRio de JaneiroBrazil
  2. 2.Department of Food EngineeringURIErechimBrazil

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