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Applied Biochemistry and Biotechnology

, Volume 162, Issue 3, pp 662–670 | Cite as

Inhibition of Pro-inflammatory Secreted Phospholipase A2 by Extracts from Cynara cardunculus L.

  • Maher Kammoun
  • Imed Koubaa
  • Yassine Ben Ali
  • Raoudha Jarraya
  • Youssef Gargouri
  • Mohamed Damak
  • Sofiane BezzineEmail author
Article

Abstract

The aim of the present work was to evaluate the anti-inflammatory properties of Cynara cardunculus L. (Asteraceae) during its growth using various solvents such as n-hexane, dichloromethane, acetone, and methanol for air-dried leaves and stems. The anti-inflammatory activities of crude extracts were evaluated by measuring the inhibition potency of mammalian non-pancreatic phospholipases A2 (hG-IIA). The methanol and acetone extracts of leaves harvested in February exhibit potent inhibition of hG-IIA (IC50 = 50 and 70 µg/ml, respectively). However, the acetone extract of stems harvested in December inhibits the hG-IIA with a lower IC50 around 130 µg/ml. Fractionation on silica gel and hydrophobic gel of the methanol extract of leaves harvested in February increases the inhibitory effect, and the IC50 reached 10 µg/ml.

Keywords

Enzyme Secreted phospholipases A2 Extraction Inhibitors Anti-inflammatory Cynara cardunculus 

Notes

Acknowledgment

This work represents a part of Maher Kammoun thesis. We are grateful to Dr. Gérard Lambeau (Institute de Pharmacologie Moléculaire et Cellulaire CNRS-Sophia Antipolis, France) for his generous supply of the recombinant hG-IIA enzyme, for fruitful discussions, and for kind help during the preparation of this work. Our thanks are also addressed to Mr R. Hamrouni for correcting the English mistakes.

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Copyright information

© Humana Press 2009

Authors and Affiliations

  • Maher Kammoun
    • 1
  • Imed Koubaa
    • 2
  • Yassine Ben Ali
    • 1
  • Raoudha Jarraya
    • 2
  • Youssef Gargouri
    • 1
  • Mohamed Damak
    • 2
  • Sofiane Bezzine
    • 1
    Email author
  1. 1.Laboratoire de Biochimie et de Génie Enzymatique des LipasesSfaxTunisia
  2. 2.Laboratoire de Chimie des Substances NaturellesFSSSfaxTunisia

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