Abstract
Batch fermentative production of fumaric acid by Rhizopus oryzae ME-F12 was investigated in a 7-l stirred tank fermentor under different dissolved oxygen (DO) concentrations. High fumaric acid yield on glucose (0.56 g/g) was achieved under high DO concentration (80%), but the glucose consumption rate and fumaric acid productivity were rather low (0.91 and 0.51 g/l/h). Fumaric acid productivity was enhanced under low DO concentration (30%), but the fuamric acid yield on glucose decreased to 0.52 g/g. In order to achieve the high fumaric acid yield and productivity simultaneously, a two-stage dissolved oxygen control strategy was proposed, in which the DO concentration was controlled at 80% in the first 18 h and then switched to 30%. This was experimentally proven to be successful. Relatively high fumaric acid production (56.2 g/l), high fumaric acid yield on glucose (0.54 g/g), and high glucose consumption rate (1.3 g/l/h) were achieved by applying this strategy. The productivity (0.7 g/l/h) was improved by 37%, 21%, and 9%, respectively, compared with fermentations in which DO concentrations were kept constant at 80%, 60%, and 30%.
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Acknowledgements
This work was supported by grants from the Ministry of Science and Technology of China (National High Technology Research and Development Program of China (No. 2006AA02Z240), National Basic Research Program of China (No. 2007CB707805)), the National Natural Science Foundation of China (No. 20706031; Grant No. 20936002), and the Natural Science Foundation of Jiangsu Province (No. BK2007186).
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Fu, YQ., Li, S., Chen, Y. et al. Enhancement of Fumaric Acid Production by Rhizopus oryzae Using a Two-stage Dissolved Oxygen Control Strategy. Appl Biochem Biotechnol 162, 1031–1038 (2010). https://doi.org/10.1007/s12010-009-8831-5
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DOI: https://doi.org/10.1007/s12010-009-8831-5