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Genome-Wide Expression Changes in Saccharomyces cerevisiae in Response to High-LET Ionizing Radiation

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Abstract

To understand the yeast response to high-linear energy transfer (LET) ionizing radiation (IR), we investigated global gene expression in yeast irradiated by three types of high-LET IR (fast neutrons, heavy ions, and thermal neutrons) and gamma rays using DNA microarray analysis. Stationary cells were irradiated by each IR and recultured in yeast–peptone–dextrose medium to allow repair for 40 min. RNA was then isolated from three independent samples of irradiated yeast. Genes involved in the Mec1p kinase pathway, which functions in DNA damage response, were induced by all forms of high-LET IR and by gamma rays. Some genes related to oxidative stress and the cell wall were induced by all forms of high-LET IRs. Gene expression patterns as a function of each type of high-LET IR were examined statistically by one-way analysis of variance. This analysis demonstrated the existence of irradiation-specific responses. For example, genes involved in ribosomal DNA synthesis were specifically induced by fast neutron irradiation, while the ubiquitin–proteasome system and heat shock response were specifically induced by thermal neutron irradiation. The study characterizes high-LET IR-induced gene expression and provides a molecular understanding of subsequent adaptation in yeast.

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Acknowledgments

This study was supported in part by the Budget for Nuclear Research of the Ministry of Education, Culture, Sports, Science and Technology, based on screening and counseling by the Atomic Energy Commission. This work was carried out in part under the Visiting Researcher's Program of the Research Reactor Institute of Kyoto University and Research Project with Heavy Ions at NIRS-HIMAC.

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Authors and Affiliations

  1. Human Stress Signal Research Center (HSS), National Institute of Advanced Industrial Science and Technology (AIST), 3A Tsukuba West, 16-1, Onogawa, Tsukuba, Ibaraki, 305-8569, Japan

    Satomi Mizukami-Murata & Hitoshi Iwahashi

  2. National Institute of Occupational Safety and Health, Kanagawa, Japan

    Shinzou Kimura

  3. National Institute of Radiological Science, Chiba, Japan

    Kumie Nojima

  4. Research Reactor Institute, Kyoto University, Osaka, Japan

    Yoshinori Sakurai, Takeshi Saitou & Noriko Fujii

  5. Japan International Research Center for Agricultural Sciences, Ibaraki, Japan

    Yoshinori Murata

  6. Hiroshima University Research Institute for Radiation Biology and Medicine, Hiroshima, Japan

    Shinzi Suga, Kazuhide Kitagawa, Kenichi Tanaka, Satoru Endo & Masaharu Hoshi

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  1. Satomi Mizukami-Murata
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  2. Hitoshi Iwahashi
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  3. Shinzou Kimura
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  4. Kumie Nojima
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  5. Yoshinori Sakurai
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Correspondence to Hitoshi Iwahashi.

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Mizukami-Murata, S., Iwahashi, H., Kimura, S. et al. Genome-Wide Expression Changes in Saccharomyces cerevisiae in Response to High-LET Ionizing Radiation. Appl Biochem Biotechnol 162, 855–870 (2010). https://doi.org/10.1007/s12010-009-8825-3

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