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Effect of Additives on the Activity of Tannase from Aspergillus awamori MTCC9299

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Abstract

Tannase from Aspergillus awamori MTCC 9299 was purified using ammonium sulfate precipitation followed by ion-exchange chromatography. A purification fold of 19.5 with 13.5% yield was obtained. Temperature of 30 °C and pH of 5.5 were found optimum for tannase activity. The effects of metals and organic solvents on the activity of tannase were also studied. Metal ions Mg+2, Mn+2, Ca+2, Na+, and K+ stimulated the tannase activity, while Cu+2, Fe+3, and Co+2 acted as inhibitors of the enzyme. The addition of organic solvents like acetic acid, isoamylalcohol, chloroform, isopropyl alcohol, and ethanol completely inhibited the enzyme activity. However, butanol and benzene increased the enzyme activity.

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References

  1. Lekha, P. K., & Lonsane, B. K. (1997). Production and application of tannin acyl hydralose: state of the art. Advances in Applied Microbiology, 44, 215–260.

    Article  CAS  Google Scholar 

  2. Mahapatra, K., Nanda, R. K., Bag, S. S., Banerjee, R., Pandey, A., & Szakacs, G. (2005). Purification, characterization and some studies on secondary structure of tannase from Aspergillus awamori nakazawa. Process Biochemistry, 40, 3251–3254.

    Article  CAS  Google Scholar 

  3. Seth, M., & Chand, S. (2000). Biosynthesis of tannase and hydrolysis of tannins to gallic acid by Aspergillus awamori—optimisation of process parameters. Process Biochemistry, 36, 39–44.

    Article  CAS  Google Scholar 

  4. Mukherjee, G., & Banerjee, R. (2006). Effects of temperature, pH and additives on the activity of tannase produced by a co-culture of Rhizopus oryzae and Aspergillus foetidus. World Journal of Microbiology & Biotechnology, 22, 207–211.

    Article  CAS  Google Scholar 

  5. Aguilar, C. N., & Gutierrez-Sanchez, G. (2001). Review: sources properties, applications and potential uses of tannin acyl hydrolase. International Journal of Food Science & Technology, 7, 373–382.

    CAS  Google Scholar 

  6. Kar, B., Banerjee, R., & Bhattacharyya, B. C. (2003). Effect of additives on the behavioural properties of tannin acyl hydrolase. Process Biochemistry, 38, 1285–1293.

    Article  CAS  Google Scholar 

  7. Saborowski, R., Sahling, G., Navarette del Toro, M. A., Walter, I., & Garcia-Carreno, F. L. (2004). Stability and effects of organic solvents on endopeptidases from thegastric fluid of the marine crab Cancer pagurus. Journal of Molecular Catalysis. B, Enzymatic, 30, 109–118.

    Article  CAS  Google Scholar 

  8. Bradoo, S., Gupta, R., & Saxena, R. K. (1996). Screening for extracellular tannase producing fungi: development of a rapid and simple plate assay. Journal of General and Applied Microbiology, 42, 325–329.

    Article  CAS  Google Scholar 

  9. Mondal, K. C., Banerjee, D., Jana, M., & Pati, B. R. (2001). Colorimetric assay method for determination of the tannase activity. Analytical Biochemistry, 295, 168–171.

    Article  CAS  Google Scholar 

  10. Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248–254.

    Article  CAS  Google Scholar 

  11. Mahendran, B., Raman, N., & Kim, D. J. (2006). Purification and characterization of tannase from Paecilomyces variotii: hydrolysis of tannic acid using immobilized tannase. Applied Microbiology and Biotechnology, 70, 444–450.

    Article  CAS  Google Scholar 

  12. Kasieczka-Burnecka, M., Karina, K., Kalinowska, H., Knap, M., & Turkiewicz, M. (2007). Purification and characterization of two cold-adapted extracellular tannin acyl hydrolases from an Antarctic strain Verticillium sp. P9. Applied Microbiology and Biotechnology, 77, 77–89.

    Article  CAS  Google Scholar 

  13. Sharma, S., Agarwal, L., & Saxena, R. K. (2008). Purification, immobilization and characterization of tannase from Penicillium variable. Biores Technology, 99, 2244–2251.

    Google Scholar 

  14. Sabu, A., Kiran, S. G., & Pandey, A. (2005). Purification and characterization of tannin acyl hydrolose from Aspergillus niger ATCC 16620. Journal of Food Technology and Biotechnology, 2, 133–138.

    Google Scholar 

  15. Kumar, R., Sharma, J., & Singh, R. (2007). Production of tannase from Aspergillus ruber under solid-state fermentation using jamun (Syzygium cumini) leaves. Microbiological Research, 162, 384–390.

    Article  CAS  Google Scholar 

  16. Rodriguez, H., Rivas, B., Gomez-Cordoves, C., & Munoz, R. (2008). Characterization of tannase activity in cell free extracts of Lactobacillus plantarun CECT748. International Journal of Food Microbiology, 121, 92–98.

    Article  CAS  Google Scholar 

  17. Zaks, A., & Klibnov, A. M. (1985). Enzyme-catalyzed processes in organic solvents. Proceedings of the National Academy of Sciences of the United States of America, 82, 3192–3196.

    Article  CAS  Google Scholar 

  18. Fridovichi, I. (1996). Some effects of organic solvents on the reaction kinetics of milk xanthine oxidase. Journal of Biological Chemistry, 241, 3624–3629.

    Google Scholar 

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Authors and Affiliations

  1. Department of Bio and Nano Technology, Guru Jambheshwar University of Science and Technology, Hisar, 125001, Haryana, India

    Vinod Chhokar & Vikas Beniwal

  2. Department of Biotechnology, Choudhary Devilal University, Sirsa, 125055, Haryana, India

    Meenakshi Sangwan & Kiran Nehra

  3. Department of Biotechnology, F.G.M. Govt. College, Adampur, 125052, Haryana, India

    Kaur S. Nehra

Authors
  1. Vinod Chhokar
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  2. Meenakshi Sangwan
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  3. Vikas Beniwal
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  4. Kiran Nehra
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  5. Kaur S. Nehra
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Correspondence to Vinod Chhokar.

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Chhokar, V., Sangwan, M., Beniwal, V. et al. Effect of Additives on the Activity of Tannase from Aspergillus awamori MTCC9299. Appl Biochem Biotechnol 160, 2256–2264 (2010). https://doi.org/10.1007/s12010-009-8813-7

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  • DOI: https://doi.org/10.1007/s12010-009-8813-7

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