Abstract
The present work explores suspicious consequence of low molecular weight glycosaminoglycan (LMW-GAG) on oxidative stress and cellular abnormalities in isoproterenol (ISO)-induced myocardial infarction in an experimental model. Group-III male Wistar rats (140 ± 10 g) were administrated by ISO (85 mg ISO/ml subcutaneously (SC) injected at the last two days of a 2–week period). Group-IV rats were treated LMW-GAG plus ISO (300 μg/day per rat SC for 1 week followed by 85 mg/kg ISO on the end last two days of the 2 - weeks). Untreated control (Group-I) and LMW-GAG drug control (Group-II) were also included. Serum and tissue lactate dehydrogenase, aminotransferases, and creatine kinase activities were increased in ISO group, which were normalized by LMW-GAG pretreatment rats. Antioxidant enzymes – superoxide dismutase (SOD), catalase and glutathione peroxidase (GPx) activities and non-enzymatic enzyme reduced glutathione (GSH) were decreased in the ISO induced rats, and this was increased by LMW-GAG pretreatment. Increased level of thiobarbituric acid reactive substances (TBARS) in plasma and the heart of ISO treated rats; pre s.c. injected with LMW-GAG to ISO-induced rats decreased the levels of TBARS. Histological examination revealed that the ISO-induced deleterious changes in the heart tissues were offset by LMW-GAG treatment. LMW-GAG affords considerable protection to the tissues challenged by cardiotoxicity, evidenced by its correction and restoration of serum and tissue indices of injury, to normalcy.
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Acknowledgements
The authors are thankful to the authorities of Annamalai University and the Director, CAS in Marine Biology for providing the facilities to carry out this work. The first author thanks the Indian Council of Medical Research, New Delhi, India for awarding Senior Research Fellowship.
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Saravanan, R., Shanmugam, A. Preventive Effect of Low Molecular Weight Glycosaminoglycan from Amussium pleuronectus (Linne) on Oxidative Injury and Cellular Abnormalities in Isoproterenol-Induced Cardiotoxicity in Wistar Rats. Appl Biochem Biotechnol 162, 43–51 (2010). https://doi.org/10.1007/s12010-009-8750-5
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DOI: https://doi.org/10.1007/s12010-009-8750-5