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A Multistage Process to Enhance Cellobiose Production from Cellulosic Materials

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Abstract

Cellobiose, a disaccharide, is a valuable product that can be obtained from cellulose hydrolysis. In this study, a simple methodology is presented to enhance the production and improve the selectivity of cellobiose during enzymatic hydrolysis of cellulose. The approach consisted of a multistage removal of filtrate via vacuum filtration and resuspension of the retentate. By this process, the remaining solid was further hydrolyzed without additional enzyme loading. Compared to the continuous hydrolysis process, the production of cellobiose increased by 45%. Increased selectivity of cellobiose is due to the loss of β-glucosidases in the filtrate, while enhanced productivity is likely due to mitigated product inhibition.

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Acknowledgements

This study was financially supported by the Walloon Region (project number 716757). We thank Ms. Virginie Byttebier for her excellent assistance.

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  1. Gembloux Agricultural University, Passage des Déportés 2, 5030, Gembloux, Belgium

    Caroline Vanderghem, Pascal Boquel, Christophe Blecker & Michel Paquot

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  1. Caroline Vanderghem
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  2. Pascal Boquel
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  3. Christophe Blecker
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  4. Michel Paquot
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Correspondence to Michel Paquot.

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Vanderghem, C., Boquel, P., Blecker, C. et al. A Multistage Process to Enhance Cellobiose Production from Cellulosic Materials. Appl Biochem Biotechnol 160, 2300–2307 (2010). https://doi.org/10.1007/s12010-009-8724-7

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  • DOI: https://doi.org/10.1007/s12010-009-8724-7

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