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Screening of Variables Influencing the Clavulanic Acid Production by Streptomyces DAUFPE 3060 Strain

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Abstract

Clavulanic acid (CA) is a β-lactam antibiotic, which has a potent β-lactamase inhibiting activity. The influence of five variables, namely pH (6.0, 6.4, and 6.8), temperature (28°C, 30°C, and 32°C), agitation intensity (150, 200, and 250 rpm), glycerol concentration (5.0, 7.5, and 10 g/L) and soybean flour concentration (5.0, 12.5, and 20 g/L), on CA production by a new isolate of Streptomyces (DAUFPE 3060) was investigated in 250-mL Erlenmeyer flasks using a fractional factorial design. Temperature and soybean flour concentration were shown to be the two variables that exerted the most important effects on the production of CA at 95% confidence level. The highest CA concentration (494 mg/L) was obtained after 48 h at 150 rpm, 32°C, pH 6.0, 5.0 g/L glycerol, and 20 g/L soybean flour concentrations. Under these conditions, the yields of biomass and product on consumed substrate were 0.26 gX/gS and 64.3 mgP/gS, respectively. Fermentations performed in 3.0-L bench-scale fermenter allowed increasing the CA production by about 60%.

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Acknowledgement

The authors gratefully acknowledge the financial support of the Brazilian Research Funding Institution FAPESP (process no. 05/60158-0).

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Authors and Affiliations

  1. Department of Biochemical and Pharmaceutical Technology, University of São Paulo, Av. Prof. Lineu Prestes, 580, Bloco 16, Cidade Universitária, 05508-000, São Paulo, São Paulo, Brazil

    D. A. Viana & A. Pessoa-Júnior

  2. LIKA, Federal University of Pernambuco, Campus Universitário, 50670-901, Recife, Pernambuco, Brazil

    M. N. Carneiro-Cunha & J. L. Lima-Filho

  3. Department of Antibiotics, Federal University of Pernambuco, Av. Prof. Luis Freire, 0740-540, Recife, Pernambuco, Brazil

    J. M. Araújo

  4. Department of Fundamental Chemistry, Federal University of Pernambuco, Av. Prof. Luis Freire, 0740-540, Recife, Pernambuco, Brazil

    B. Barros-Neto

  5. Department of Chemical and Process Engineering, University of Genoa, Via Opera Pia 15, 16145, Genoa, Italy

    A. Converti

  6. Department of Animal Morphology and Physiology, Federal Rural University of Pernambuco, Av. Dom Manoel de Medeiros, s/n, 52171-900, Dois Irmãos, Pernambuco, Brazil

    A. L. F. Porto

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  1. D. A. Viana
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  2. M. N. Carneiro-Cunha
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  3. J. M. Araújo
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  4. B. Barros-Neto
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  5. J. L. Lima-Filho
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  6. A. Converti
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  7. A. Pessoa-Júnior
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  8. A. L. F. Porto
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Correspondence to A. Converti.

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Viana, D.A., Carneiro-Cunha, M.N., Araújo, J.M. et al. Screening of Variables Influencing the Clavulanic Acid Production by Streptomyces DAUFPE 3060 Strain. Appl Biochem Biotechnol 160, 1797–1807 (2010). https://doi.org/10.1007/s12010-009-8671-3

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  • DOI: https://doi.org/10.1007/s12010-009-8671-3

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