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Bleach Enhancement of Mixed Wood Pulp by Xylanase–Laccase Concoction Derived Through Co-culture Strategy

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Abstract

Mixed enzyme preparation having both xylanase and laccase activity was evaluated for its bleach enhancing ability of mixed wood pulp. The enzyme was produced through co-cultivation of mutant Penicillium oxalicum SAUE-3.510 and Pleurotus ostreatus MTCC 1804 under solid-state fermentation. Bleaching of pulp with mixed enzyme had resulted into a notable decrease in kappa number and increased brightness as compared to xylanase alone. Analysis of bleaching conditions had denoted that 8 IU g−1 of mixed enzyme preparation (xylanase/laccase, 22:1) had led into maximal removal of lignin from pulp when bleaching was performed at 10% pulp consistency (55 °C, pH 9.0) for 3 h. An overall improvement of 21%, 8%, 3%, and 5% respectively in kappa number, brightness, yellowness, and viscosity of pulp was achieved under derived bleaching conditions. Process of enzymatic bleaching was further ascertained by analyzing the changes occurring in polysaccharide and lignin by HPLC and FTIR. The UV absorption spectrum of the compounds released during enzymatic treatment had denoted a characteristic peak at 280 nm, indicating the presence of lignin in released coloring matter. The changes in fiber morphology following enzymatic delignification were studied by scanning electron microscopy.

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Acknowledgements

Senior research fellowship awarded by Council of Scientific and Industrial Research, New Delhi, India to first author and national doctoral fellowship to second author by All India Council of Technical Education, New Delhi, India are gratefully acknowledged.

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  1. Pallavi Dwivedi

    Present address: Department of Biotechnology, Sir Padampat Singhania University, Udaipur, 313601, India

  2. V. Vivekanand

    Present address: Lundberg Laboratory, CMB-Molecular Biology, Gothenburg University, Gothenburg, 40530, Sweden

  3. Amit Sharma

    Present address: Division of Genetics, Indian Agricultural Research Institute, New Delhi, 110 012, India

Authors and Affiliations

  1. Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, 247 667, India

    Pallavi Dwivedi, V. Vivekanand, Nidhi Pareek, Amit Sharma & Rajesh P. Singh

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Dwivedi, P., Vivekanand, V., Pareek, N. et al. Bleach Enhancement of Mixed Wood Pulp by Xylanase–Laccase Concoction Derived Through Co-culture Strategy. Appl Biochem Biotechnol 160, 255–268 (2010). https://doi.org/10.1007/s12010-009-8654-4

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