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Transgenic Soybean Seed as Protein Expression System: Aqueous Extraction of Recombinant β-Glucuronidase

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Abstract

Soybean is one of the plant species with potential to be used as seed-based bioreactor. As part of the downstream processing (DSP) of this technology, extraction is a key step, since it defines the composition of the solution from which the recombinant product will be purified. In the present work, the characteristics of soybean seeds used as a bioreactor were evaluated from a process engineering standpoint through analysis of the influence of pH and ionic strength on the extraction of recombinant β-glucuronidase (rGUS). Concentrations of recombinant protein and native soybean compounds were analyzed and compared with similar data from extraction studies using transgenic corn seeds as bioreactor. Efficient rGUS extraction was obtained at pH of around 5.5 with no addition of salt. Soybean seed extracts had lower levels of co-extracted native compounds, than corn seed extracts, and should be considered as a potential plant bioreactor in terms of DSP.

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Acknowledgments

The authors would like to thank CNPq (Brazil) for its financial support.

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Authors and Affiliations

  1. LEBp: Laboratório de Engenharia de Bioprocessos, Departamento de Processos Biotecnológicos, Faculdade de Engenharia Química, Universidade Estadual de Campinas, CP 6066, CEP 13083-970, Campinas, SP, Brazil

    Goran Robić & Everson A. Miranda

  2. Embrapa Instrumentação Agropecuária, Rua 15 de Novembro, 1452, CEP 13560-970, São Carlos, SP, Brazil

    Cristiane S. Farinas

  3. Laboratório de Transferência de Genes, Embrapa Recursos Genéticos e Biotecnologia, CP 02372, CEP 70770-900, Brasília, DF, Brazil

    Elíbio L. Rech

Authors
  1. Goran Robić
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  2. Cristiane S. Farinas
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  3. Elíbio L. Rech
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  4. Everson A. Miranda
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Correspondence to Everson A. Miranda.

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Robić, G., Farinas, C.S., Rech, E.L. et al. Transgenic Soybean Seed as Protein Expression System: Aqueous Extraction of Recombinant β-Glucuronidase. Appl Biochem Biotechnol 160, 1157–1167 (2010). https://doi.org/10.1007/s12010-009-8637-5

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  • DOI: https://doi.org/10.1007/s12010-009-8637-5

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