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Semi-continuous Cultivation of Haematococcus pluvialis for Commercial Production

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Abstract

The objectives of the present study on the growth of Haematococcus pluvialis were to indicate the effects of a long-term semi-continuous cultivation, sterilization, carbon dioxide, and different culture media by using artesian well water. This investigation was an enterprise in order to commercialize the production economically. When the effect of CO2 was investigated in basal culture medium, the influence of sterilization was also researched in Rudic’s culture medium in vertical panel-type photobioreactors for 31 days of semi-continuous cultivation. The maximum cell concentration of 10.55 × 105 cells ml−1, which corresponds to the growth rate of 0.271 day−1 with the areal productivity of 3.531 g m−2 day−1, was found in non-sterilized RM medium on the 24th day of the third run of semi-continuous cultivation at a renewal rate of 50% in a vertical panel-type photobioreactor.

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Abbreviations

C b :

Biomass concentration (g l−1)

C c :

Cell count (cells ml−1)

DT:

Doubling time (day)

P ba :

Areal productivity (g m−2 day−1)

P bv :

Volumetric productivity (g l−1 day−1)

µ :

Specific growth rate (day−1)

References

  1. Richmond, A. (2004). Handbook of microalgal culture: Biotechnology and applied phycology (2nd ed.), pp. 281–288. Iowa, USA: Iowa State Press.

    Google Scholar 

  2. Hagen, C., Grünewald, K., Xylander, M., & Rothe, E. (2001). Journal of Applied Phycology, 13, 79–87.

    Article  Google Scholar 

  3. Chen, F. (1996). Trends in Biotechnology, 14, 421–426.

    Article  CAS  Google Scholar 

  4. Javanmardian, M., & Palsson, B. O. (1991). Biotechnology and Bioengineering, 38, 1182–1189.

    Article  CAS  Google Scholar 

  5. Chen, F., & Johns, M. R. (1995). Journal of Applied Phycology, 7, 43–46.

    Article  CAS  Google Scholar 

  6. Chen, F., Chen, H., & Gong, X. (1997). Bioresource Technology, 62, 19–24.

    Article  CAS  Google Scholar 

  7. Margalith, P. (1999). Applied Microbiology Biotechnology, 51, 431–438.

    Article  CAS  Google Scholar 

  8. Dalay, M. C. (2003). In M. C. Dalay (Ed.), International workshop and training course on photobioreactor, vol. 1: An introduction to microalgal biotechnology and role of photobioreactors, pp. 6–7. Izmir, Turkey: Ebiltem.

    Google Scholar 

  9. Rippka, R., Deruelles, J. B., Herdman, M., & Stanier, R. Y. (1979). Journal of General Microbiology, 111, 1–61.

    Google Scholar 

  10. Torzillo, G., Goksan, T., Faraloni, C., Kocky, J., & Masojidek, J. (2003). Journal of Applied Phycology, 15, 127–136.

    Article  CAS  Google Scholar 

  11. Hata, N., Ogbonna, J., Hasegawa, Y., Taroda, H., & Tanaka, H. (2001). Journal of Applied Phycology, 13, 395–402.

    Article  CAS  Google Scholar 

  12. Rudic, V., & Dudnicenco, T. (2000) MD Patent Nr. a 2000 0154.

  13. Molina, E., Fernández, J., Acién, F. G., & Chisti, Y. (2001). J. Biotechnologies, 92, 113–131.

    CAS  Google Scholar 

  14. Fàbregas, J., Otero, A., Maseda, A., & Domìnguez, A. (2001). Journal of Biotechnology, 89, 65–71.

    Article  Google Scholar 

  15. Kaewpintong, K., Shotipruk, A., Powtongsook, S., & Pavasant, P. (2007). Bioresource Technology, 98, 288–295.

    Article  CAS  Google Scholar 

  16. Ranjbar, R., Inoue, R., Shiraishi, H., Katsuda, T., & Katoh, S. (2007). Biochemical Engineering Journal, 39, 575–580.

    Google Scholar 

  17. García-Malea López, M. C., Del Río Sánchez, E., Casas López, J. L., Acíen Fernandez, F. G., Fernández Sevilla, J. M., Rivas, J., et al. (2006). Journal of Biotechnology, 123, 329–342.

    Article  Google Scholar 

  18. Becker, E. W. (1995). In E. W. Becker (Ed.), Microalgae: Biotechnology and microbiology, vol. 2: Culture media, pp. 9–42. Cambridge: Cambridge University Press.

    Google Scholar 

  19. Boussiba, S., Vonshak, A., Cohen, Z., Richmond, A. (2000) US Patent 6,022,701.

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Acknowledgment

We wish to thank Ege University Scientific Research Fund for financial support.

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Correspondence to Esra Imamoglu.

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Imamoglu, E., Dalay, M.C. & Sukan, F.V. Semi-continuous Cultivation of Haematococcus pluvialis for Commercial Production. Appl Biochem Biotechnol 160, 764–772 (2010). https://doi.org/10.1007/s12010-009-8627-7

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