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Water Activity Dependence of Lipases in Non-aqueous Biocatalysis

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Abstract

Eleven lipases are tested and it was found that lipases can be divided into three types according to water activity dependence. The first type is lipase that has low water activity dependence and works in a low water activity, its performance changes little with the change of water activity. The optimum water activity is 0.19 and Newlase F (Rhizopus niveus), lipase FAP-15 (Rhizopus oryzae) belong to this type. The second type is lipase that has medium water activity dependence and its performance changes with the change of water activity. Most lipases belong to this type and the optimum water activity in this type is about 0.60. The third type is lipase that has a high water activity dependence and works only in a high water activity (a w  > 0.75). WGL (wheat germ) belongs to this type and the optimum water activity is 0.90. The relationship between enantioselectivity and water activity is also discussed and the enantioselectivity seems to be independent of water activity. And we also compared the two control methods of water activity, it was found that the method which add solid salt hydrates to the reaction mixture (method II) is more stable and effective throughout the reaction than the method that pre-equilibrate via the vapor phase (method I). The addition concentration of salt hydrates is also investigated and the optimum concentration is 1 g/l.

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Acknowledgement

This work was supported by National Natural Science Foundation of China (20506007 and 20706021). We are grateful to Amano enzyme Inc. for the generous gift of enzymes used in this study. And we wish to thank the suggestion from Professor C.G. Whiteley.

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Authors and Affiliations

  1. School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510641, China

    Xiaole Xia, Chen Wang, Bo Yang & Xiaoning Wang

  2. College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, 510641, China

    Yong-Hua Wang

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  1. Xiaole Xia
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  2. Chen Wang
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  3. Bo Yang
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  4. Yong-Hua Wang
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  5. Xiaoning Wang
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Correspondence to Yong-Hua Wang or Xiaoning Wang.

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Xia, X., Wang, C., Yang, B. et al. Water Activity Dependence of Lipases in Non-aqueous Biocatalysis. Appl Biochem Biotechnol 159, 759–767 (2009). https://doi.org/10.1007/s12010-009-8618-8

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  • DOI: https://doi.org/10.1007/s12010-009-8618-8

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