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Antibacterial Activities of Crude Extract of Aloe barbadensis to Clinically Isolated Bacterial Pathogens

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Abstract

The antibacterial activity of Aloe barbadensis was tested on clinically isolated bacterial pathogens i.e. Enterococcus bovis, Staphylococcus aureus, Escherichia coli, Proteus vulgaris, Proteus mirabilis, Pseudomonas aeruginosa, Morganella morganii, and Klebsiella pneumoniae causing infection in human being. Ethanolic and aqueous extracts were used for the antibacterial effect, which was measured by the appearance of zone of inhibition. Relatively higher MIC concentrations were obtained for gram negative bacteria E. coli and K. pneumoniae, with ethanol extract; however, no inhibitory effect was noted for aqueous extract. Ethanolic extract possesses great inhibitory activity for gram positive bacteria, E. bovis followed by S. aureus. Among gram negative bacteria, highest inhibitory effect was observed with P. aeruginosa, followed by M. morganii, P. mirabilis, and P. vulgaris, which was significant (p < 0.01) than E. coli and K. pneumoniae. Antimicrobial activity tests of crude extract of A. barbadensis were carried out to validate the use of traditional medicinal herbal and results of this study tend to give credence to the common use of A. barbadensis gel and leaf.

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Acknowledgement

The authors are grateful to the Head, Department of Medical Biochemistry and Secretary, Uttarakhand Forest Hospital Trust Medical College, Haldwani, India for their support, encouragement and providing research facilities. Department of Medical Microbiology, Uttarakhand Forest Hospital Trust Medical College, Haldwani, India is duly acknowledged for providing pure culture of clinically isolated bacterial pathogen.

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  1. Avinash Mishra

    Present address: Molecular Biology and Biotechnology, CSMCRI, Bhavnagar, 364002, Gujarat, India

Authors and Affiliations

  1. Department of Biotechnology, Institute of Allied Health (Paramedical) Services, Education and Training, Uttarakhand Forest Hospital Trust, Rampur Road, Haldwani, 263139, Uttarakhand, India

    Ruchi Pandey

  2. G. B. Pant University of Agriculture and Technology, Pantnagar, 263145, Uttarakhand, India

    Avinash Mishra

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  1. Ruchi Pandey
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  2. Avinash Mishra
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Correspondence to Avinash Mishra.

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Pandey, R., Mishra, A. Antibacterial Activities of Crude Extract of Aloe barbadensis to Clinically Isolated Bacterial Pathogens. Appl Biochem Biotechnol 160, 1356–1361 (2010). https://doi.org/10.1007/s12010-009-8577-0

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