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Structural Characterization of Lipopeptides from Brevibacillus brevis HOB1

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Abstract

Brevibacillus brevis HOB1 was isolated from the formation water of an oil field and found to produce lipopeptides. The separation of lipopeptides was successfully achieved by reversed-phase high-performance liquid chromatography (HPLC) leading to nine separated peaks. The chemical structures of these lipopeptides were studied by means of electrospray ionization mass spectrometry (ESI-MS), gas chromatography-mass spectrometry (GC/MS), HPLC and electrospray ionization tandem mass spectrometry (ESI-MS/MS). As the results, all the lipopeptides had peptide parts with the same amino acid composition of Asp, Glu, Val, and Leu in the molar ratio 1:1:1:4, while the lipid part was composed of C13–C15β-hydroxy fatty acids. As the sequence of fraction 1 was determined to be N-Glu-Leu-Leu-Val-Asp-Leu-Leu-C, the same as surfactin, they were proposed to be surfactin isoforms. Fraction 4 (C15 surfactin) exhibited a good surface activity of 26.8 mN/m with CMC of 9 × 10−6 M. Surfactin is a powerful biosurfactant possessing biological activities. As far as we know, Br. brevis is a new surfactin-producing species.

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Acknowledgement

This work was supported by the National Natural Science Foundation of China (No.50744016), the grant from the Ministry of Science and Technology of China (2007CB707801), and Shanghai Municipal Science and Technology Commission (071607014).

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  1. State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, Shanghai, 200237, China

    Ji Wang, Namir I. A. Haddad, Shi-Zhong Yang & Bo-Zhong Mu

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  1. Ji Wang
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  2. Namir I. A. Haddad
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  3. Shi-Zhong Yang
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Correspondence to Bo-Zhong Mu.

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Wang, J., Haddad, N.I.A., Yang, SZ. et al. Structural Characterization of Lipopeptides from Brevibacillus brevis HOB1. Appl Biochem Biotechnol 160, 812–821 (2010). https://doi.org/10.1007/s12010-009-8536-9

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