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Enhanced Production of Spinosad in Saccharopolyspora spinosa by Genome Shuffling

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Abstract

Spinosad (spinosyns A and D) is a mixture of secondary metabolites produced by Saccharopolyspora spinosa. It is used in agriculture as a potent insect control agent with exceptional safety to non-target organisms. In this study, we applied genome shuffling of S. spinosa to achieve a rapid improvement of spinosad production. Ten strains with subtle improvements in spinosad production were obtained from the populations generated by the mutation with nitrosoguanidine and ultraviolet irradiation, and then they were subjected for recursive protoplast fusion. After four rounds of genome shuffling, a high yielding strain, designated as S. spinosa 4-7, was successfully isolated. Its production reached 547 mg/L, which was increased by 200.55% and 436.27% in comparison with that of the highest parent strain and the original strain, respectively. The subculture experiments indicated that the high producer of S. spinosa 4-7 was stable. Spinosad fermentation experiments by S. spinosa 4-7 were carried out in a 5-L fermentor, and its production of spinosad reached 428 mg/L after 168 h of fermentation.

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Acknowledgments

This work was financially supported by the Natinal Basic Research Program (973) of China (no. 2007CB714305), the National Natural Science Foundation of China (no. 20576122), and the Natural Science Foundation of Zhejiang Province, China (no. Y404291).

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Authors and Affiliations

  1. Department of Biochemical and Pharmaceutical Engineering, Ningbo Institute of Technology, Zhejiang University, 315100, Ningbo, People’s Republic of China

    Z. H. Jin, B. Xu, S. Z. Lin & Q. C. Jin

  2. Institute of Bioengineering, College of Materials Science and Chemical Engineering, Zhejiang University, 310027, Hangzhou, People’s Republic of China

    Z. H. Jin, B. Xu & P. L. Cen

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  1. Z. H. Jin
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  2. B. Xu
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  3. S. Z. Lin
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  4. Q. C. Jin
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  5. P. L. Cen
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Correspondence to Z. H. Jin.

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Jin, Z.H., Xu, B., Lin, S.Z. et al. Enhanced Production of Spinosad in Saccharopolyspora spinosa by Genome Shuffling. Appl Biochem Biotechnol 159, 655–663 (2009). https://doi.org/10.1007/s12010-008-8500-0

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  • DOI: https://doi.org/10.1007/s12010-008-8500-0

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