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Biodiesel Production from Various Oils Under Supercritical Fluid Conditions by Candida antartica Lipase B Using a Stepwise Reaction Method

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Abstract

In this study, we evaluate the effects of various reaction factors, including pressure, temperature, agitation speed, enzyme concentration, and water content to increase biodiesel production. In addition, biodiesel was produced from various oils to establish the optimal enzymatic process of biodiesel production. Optimal conditions were determined to be as follows: pressure 130 bar, temperature 45 °C, agitation speed 200 rpm, enzyme concentration 20%, and water contents 10%. Among the various oils used for production, olive oil showed the highest yield (65.18%) upon transesterification. However, when biodiesel was produced using a batch system, biodiesel conversion yield was not increased over 65%; therefore, a stepwise reaction was conducted to increase biodiesel production. When a reaction medium with an initial concentration of methanol of 60 mmol was used and adjusted to maintain this concentration of methanol every 1.5 h during biodiesel production, the conversion yield of biodiesel was 98.92% at 6 h. Finally, reusability was evaluated using immobilized lipase to determine if this method was applicable for industrial biodiesel production. When biodiesel was produced repeatedly, the conversion rate was maintained at over 85% after eight reuses.

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Acknowledgements

This study was supported by research grants from the Korea Science and Engineering Foundation (KOSEF) through the Applied Rheology Center (ARC), an official KOSEF-created engineering research center (ERC) at Korea University, Seoul, Korea, and the Brain Korea 21 program issued by the Ministry of Education, Korea.

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Authors and Affiliations

  1. Department of Chemical and Biological Engineering, Korea University, 1, Anam-dong, Sungbuk-ku, Seoul, 136-701, Korea

    Jong Ho Lee, Cheong Hoon Kwon, Jeong Won Kang & Seung Wook Kim

  2. Department of Chemical Engineering, Kwangwoon University, 447-1, Wolgye-Dong, Nowon-Gu, Seoul, 139-701, Korea

    Chulhwan Park

  3. Department of Chemical Engineering, Hankyong National University, 67 Sukjung-dong, Ansung-city, Kyonggi-do, 456-749, Korea

    Bumseok Tae

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  1. Jong Ho Lee
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  2. Cheong Hoon Kwon
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Correspondence to Seung Wook Kim.

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Lee, J.H., Kwon, C.H., Kang, J.W. et al. Biodiesel Production from Various Oils Under Supercritical Fluid Conditions by Candida antartica Lipase B Using a Stepwise Reaction Method. Appl Biochem Biotechnol 156, 24–34 (2009). https://doi.org/10.1007/s12010-008-8488-5

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  • DOI: https://doi.org/10.1007/s12010-008-8488-5

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