Abstract
This study evaluated the production of cellulolytic enzymes by an Aspergillus fumigatus strain, isolated from sugar cane bagasse, according to its ability to grow on microcrystalline cellulose as the sole carbon source. The effect of the carbon source (brewer’s spent grain, sugarcane bagasse, and wheat bran) and of the nitrogen source (corn steep liquor and sodium nitrate) on cellulase production was studied using submerged and solid state cultivations at 30 °C. The highest levels of endoglucanase (CMCase) corresponded to 365 U L-1 and was obtained using sugarcane bagasse (1%) and corn steep liquor (1.2%) in submerged fermentation within 6 days of cultivation. This supernatant was used to run a sodium dodecyl sulfate polyacrylamide gel electrophoresis that showed six bands with endoglucanase activity. CMCase activity was higher at 65 °C and pH 2.0, indicating that this microorganism produces a thermophilic and acid endoglucanase. Solid state cultivation favored FPase production, that reached 47 U g-1 of dry substrate (wheat bran and sugarcane bagasse) within 3 days.
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Acknowledgments
Authors are indebted to Marta de Sousa Ferreira for technical support and Fundação Oswaldo Cruz for identification of the fungal strain. This work was financially supported by CNPq and FINEP (project no 0106004700).
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Grigorevski-Lima, A.L., Da Vinha, F.N.M., Souza, D.T. et al. Aspergillus fumigatus Thermophilic and Acidophilic Endoglucanases. Appl Biochem Biotechnol 155, 18–26 (2009). https://doi.org/10.1007/s12010-008-8482-y
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DOI: https://doi.org/10.1007/s12010-008-8482-y