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Bioremediation of Marine Sediments Impacted by Petroleum

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Abstract

The aim of this work was to optimize the bioremediation of crude oil-contaminated sand sediment through the biostimulation technique. The soil was obtained in the mid-tide zone of Guanabara Bay, Rio de Janeiro, Brazil and was artificially contaminated with crude oil at 14 g kg−1. Bioremediation optimization was performed using an experimental design and statistical analysis of the following factors: supplementation with commercial biosurfactant Jeneil® IBR 425 and commercial mineral NPK fertilizer. The response variable used was the biodegradation of the heavy oil fraction, HOF. The analysis of the studied factors and their interactions was executed using contour plots, Pareto diagram and ANOVA table. Experimental design results indicated that the supplementation with fertilizer at 100:25:25 C/N/P ratio and biosurfactant at 2 g kg−1 yielded biodegradation of HOF at about 30% during 30 days of process. Some experiments were carried out using the experimental design results, yielding 65% of biodegradation of HOF and 100% of n- alkanes between C15 and C30 during 60 process days. Intrinsic biodegradation test was carried out, yielding 85% of biodegradation of n-alkanes between C15 and C30 during 30 days of process.

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Acknowledgments

Our thanks to Coordenação de Apoio a Pessoal de Nível Superior, CAPES, Conselho Nacional de Desenvolvimento Científico e Tecnológico, CNPq, and Financiadora de Projetos, FINEP, for the financial support. The authors would like to thank Petrobras for providing the Arabian light oil employed in this research.

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Authors and Affiliations

  1. Escola de Química, Universidade Federal do Rio de Janeiro, Centro de Tecnologia, Bloco E, Ilha do Fundão, 21949-900, Rio de Janeiro, RJ, Brazil

    Aike C. da Silva, Diogo S. Bernardes & Francisca P. de França

  2. Petróleo Brasileiro SA, Avenida Almirante Barroso, 81, 24° andar, Centro, 20031-004, Rio de Janeiro, RJ, Brazil

    Fernando J. S. de Oliveira

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  1. Aike C. da Silva
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  2. Fernando J. S. de Oliveira
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  3. Diogo S. Bernardes
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  4. Francisca P. de França
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Correspondence to Aike C. da Silva.

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da Silva, A.C., de Oliveira, F.J.S., Bernardes, D.S. et al. Bioremediation of Marine Sediments Impacted by Petroleum. Appl Biochem Biotechnol 153, 58–66 (2009). https://doi.org/10.1007/s12010-008-8457-z

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