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Endoxanthanase, a Novel β-d-Glucanase Hydrolyzing Backbone Linkage of Intact Xanthan from Newly Isolated Microbacterium sp. XT11

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Abstract

A novel endoxanthanase was produced and isolated from the culture of Microbacterium sp. XT11 growing on xanthan. Optimum pH and temperature for the enzyme activity were 6.0 and 35–40 °C, respectively. The endoxanthanase cleaves the backbone endo-β-1,4-glucosidic linkage of the xanthan molecule, which is specific to the intact xanthan. However, the lyase-treated xanthan or carboxymethyl cellulose could not be cleaved by endoxanthanase.

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Acknowledgments

The scientific research was supported financially by Natural and Scientific Funding of China, Liaoning Scientific Research Foundation, and Research Funding from Education Department of Liaoning.

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Authors and Affiliations

  1. Department of Bio & Food Engineering, Dalian College of Light Industry, Dalian, 116034, People’s Republic of China

    Bing Li, Jiqiang Guo, Xiaoyi Chen, Ligang Chen, Zhiwen Liu & Xianzhen Li

  2. Institute of Rice Culture, Shenyang Agricultural University, Shenyang, 110161, People’s Republic of China

    Wenfu Chen

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  1. Bing Li
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  2. Jiqiang Guo
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  3. Wenfu Chen
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  4. Xiaoyi Chen
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  5. Ligang Chen
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  7. Xianzhen Li
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Correspondence to Xianzhen Li.

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Li, B., Guo, J., Chen, W. et al. Endoxanthanase, a Novel β-d-Glucanase Hydrolyzing Backbone Linkage of Intact Xanthan from Newly Isolated Microbacterium sp. XT11. Appl Biochem Biotechnol 159, 24–32 (2009). https://doi.org/10.1007/s12010-008-8439-1

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  • DOI: https://doi.org/10.1007/s12010-008-8439-1

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