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Enhanced Production of Poly (γ-glutamic acid) from Bacillus licheniformis NCIM 2324 by Using Metabolic Precursors

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Abstract

The aim of the present work was to study the effect of addition of different amino acids and tricarboxylic acid cycle intermediates as metabolic precursors on the production of poly (γ-glutamic acid) (PGA) by Bacillus licheniformis NCIM 2324. A maximum yield of 35.75 g/l was obtained with the medium supplemented with 0.5 mM l-glutamine and 10 mM α-ketoglutaric acid as compared to 26.12 g/l PGA achieved with the control in the absence of metabolic precursors. Addition of precursors also enhanced the utilization of l-glutamic acid during fermentation.

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Authors and Affiliations

  1. Food Engineering and Technology Department, Institute of Chemical Technology, University of Mumbai, Matunga, Mumbai, 400 019, India

    Ishwar B. Bajaj & Rekha S. Singhal

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  1. Ishwar B. Bajaj
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  2. Rekha S. Singhal
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Correspondence to Ishwar B. Bajaj.

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Bajaj, I.B., Singhal, R.S. Enhanced Production of Poly (γ-glutamic acid) from Bacillus licheniformis NCIM 2324 by Using Metabolic Precursors. Appl Biochem Biotechnol 159, 133–141 (2009). https://doi.org/10.1007/s12010-008-8427-5

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