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Degradation of Phytates in Distillers’ Grains and Corn Gluten Feed by Aspergillus niger Phytase

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Abstract

Distillers’ dried grains with solubles (DDGS) and corn gluten feed (CGF) are major coproducts of ethanol production from corn dry grind and wet milling facilities, respectively. These coproducts contain important nutrients and high levels of phytates. The phytates in these products cannot be digested by nonruminant animals; consequently, large quantities of phytate phosphorus (P) are deposited into the soil with the animal wastes which potentially could cause P pollution in soil and underground water resources. To reduce phytates in DDGS and CGF, a phytase from Aspergillus niger, PhyA, was investigated regarding its capability to catalyze the hydrolysis of phytates in light steep water (LSW) and whole stillage (WS). LSW and WS streams are the intermediate streams in the production of CGF and DDGS, respectively, and contribute to most of the P in these streams. Enzyme loadings with activity of 0.1, 1, 2, and 4 FTU/g substrate and temperatures of 35 and 45 °C were investigated regarding their influences on the degree of hydrolysis. The analysis of the hydrolyzate suggested to a sequentially degradation of phytates to lower order myo-inositol phosphate isomers. Approximately 90% phytate P of LSW and 66% phytate P of WS were released, suggesting myo-inositol monophosphate as the end product. The maximum amount of released P was 4.52 ± 0.03 mg/g LSW and 0.86 ± 0.01 mg/g WS.

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References

  1. Correll, D. L. (1999). Poultry Science, 78, 674–682.

    CAS  Google Scholar 

  2. Rausch, K. D., & Belyea, R. L. (2006). Applied Biochemistry and Biotechnology, 128, 47–86. doi:10.1385/ABAB:128:1:047.

    Article  CAS  Google Scholar 

  3. EPA (1998). National water quality inventory: 1996 Report to Congress. Washington, DC: Office of Water, EPA EPA, 841-R-97-008, June.

    Google Scholar 

  4. Parry, R. (1998). Journal of Environmental Quality, 27, 258–261.

    CAS  Google Scholar 

  5. Sharpley, A., Daniel, T. C., Sims, J. T., & Pote, D. H. (1996). Journal of Soil and Water Conservation, 51, 160–166.

    Google Scholar 

  6. McDowell, R. W., & Sharpley, A. N. (2001). Journal of Environmental Quality, 30, 508–520.

    Article  CAS  Google Scholar 

  7. RFA. (2006), Ethanol industry outlook. Available from: www.ethanolrfa.org/industry/resources/coproducts.

  8. Rausch, K. D., Raskin, L. M., Belyea, R. L., Agbisit, R. M., Daugherty, B. J., Clevenger, T. E., et al. (2005). Cereal Chemistry, 82, 431–435. doi:10.1094/CC-82-0431.

    Article  CAS  Google Scholar 

  9. Erickson, G. E., Auvermann, B., Eigenberg, R., Greene, L. W., Klopfenstein, T., & Koelsch, R. (2003). Proceedings of the Ninth International Symposium on Animal, Agricultural and Food Processing Wastes (pp 269–276), St. Joseph.

  10. Koelsch, R., & Lesoing, G. (1999). Journal of Animal Science, 77(Suppl. 2), 63–71.

    CAS  Google Scholar 

  11. Reddy, N. R. (2002). In N. R. Reddy & S. K. Sath (Eds.), Food phytates (pp. 25–52). Boca Raton: CRC.

    Google Scholar 

  12. Angel, R., Tamim, N. M., Applegate, T. J., Ellestad, L. E., & Dhandu, A. S. (2002). Journal of Applied Poultry Research, 11, 471–480.

    CAS  Google Scholar 

  13. Ravindran, V., Bryden, W. L., & Kornegay, E. T. (1995). Poultry and Avian Biology Reviews, 6, 125–143.

    Google Scholar 

  14. Cromwell, G. L., Coeffy, R. D., Monegue, H. J., & Randolph, J. H. (1995). Journal of Animal Science, 73, 449–458.

    CAS  Google Scholar 

  15. Cowieson, A. J., Acamovic, T., & Bedford, M. R. (2006). Poultry Science, 85, 878–885.

    CAS  Google Scholar 

  16. Nelson, T. S., Sheih, T. R., Wodzinski, R. J., & Ware, J. H. (1971). The Journal of Nutrition, 101, 1289–1294.

    CAS  Google Scholar 

  17. Simons, P. C. M. A., Versteegh, H. A. J., Jongbloed, A. W., Kemme, P. A., Slump, P., Bos, K. D., et al. (1990). Brit. J. Nutr, 64, 525–540. doi:10.1079/BJN19900052.

    Article  CAS  Google Scholar 

  18. Cosgrove, D. J. (1980a). Studies in organic chemistry 4 (pp. 85–98). Amsterdam: Elsevier.

    Google Scholar 

  19. Cosgrove, D. J. (1980b). Studies in organic chemistry 4 (pp. 99–105). Amsterdam: Elsevier.

    Google Scholar 

  20. Wyss, M., Pasamontes, L., Friedlein, A., Rémy, R., Tessier, M., Kronenberger, A., et al. (1999a). Applied and Environmental Microbiology, 65, 367–373.

    CAS  Google Scholar 

  21. Wyss, M., Pasamontes, L., Friedlein, A., Rémy, R., Tessier, M., Kronenberger, A., et al. (1999b). Applied and Environmental Microbiology, 65, 359–366.

    CAS  Google Scholar 

  22. Gibson, D. M., & Ullah, A. H. (1988). Archives of Biochemistry and Biophysics, 260, 503–513. doi:10.1016/0003-9861(88)90475-4.

    Article  CAS  Google Scholar 

  23. Barrientos, L., Scott, J. J., & Murthy, P. P. (1994). Plant Physiology, 106, 1489–1495.

    Article  CAS  Google Scholar 

  24. Shieh, T. R., & Ware, J. H. (1968). Applied Microbiology, 16, 1348–1351.

    CAS  Google Scholar 

  25. Shieh, T. R., Wodzinski, R. J., & Ware, J. H. (1969). Journal of Bacteriology, 100, 1161–1165.

    CAS  Google Scholar 

  26. Ullah, A. H., & Sethumadhavan, K. (1998). Biochemical and Biophysical Research Communications, 243, 458–462. doi:10.1006/bbrc.1998.8117.

    Article  CAS  Google Scholar 

  27. Abelson, P. H. (1999). Science, 283, 2015. doi:10.1126/science.283.5410.2015.

    Article  CAS  Google Scholar 

  28. Kim, T., Mullaney, E. J., Porres, J. M., Roneker, K. R., Crowe, S., Rice, S., et al. (2006). Applied and Environmental Microbiology, 72, 4397–4403. doi:10.1128/AEM.02612-05.

    Article  CAS  Google Scholar 

  29. Cromwell, G. L., Stahly, T. S., Coffey, R. D., Monegue, H. J., & Randolph, J. H. (1993). Journal of Animal Science, 71, 1831–1840.

    CAS  Google Scholar 

  30. Wodzinski, R. J., & Ullah, A. H. (1996). Advances in Applied Microbiology, 42, 263–302. doi:10.1016/S0065-2164(08)70375-7.

    Article  CAS  Google Scholar 

  31. Noureddini, H. Malik, M., Byun, J., & Ankeny, A. J. (2008). Bioresource Technology, doi:10.1016/j.biortech.2008.05.056.

  32. Engelen, A. J., van der Heeft, F. C., Randsdorp, P. H. G., & Smit, E. L. C. (1994). Journal of AOAC International, 77, 760–764.

    CAS  Google Scholar 

  33. Pulliaine, T. K., & Wallin, H. C. (1994). Journal of AOAC International, 77, 1557–1561.

    Google Scholar 

  34. Chen, Q. C., & Betty, W. L. (2003). Journal of Chromatography. A, 1018, 41–52. doi:10.1016/j.chroma.2003.08.040.

    Article  CAS  Google Scholar 

  35. Xu, P., Price, J., & Aggett, P. J. (1992). Progress in Food & Nutrition Science, 16, 245–262.

    CAS  Google Scholar 

  36. Lee, D. C., Cottrill, M. A., Forsberg, C. W., & Jia, Z. C. (2003). The Journal of Biological Chemistry, 278, 31412–31418. doi:10.1074/jbc.M213154200.

    Article  CAS  Google Scholar 

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Acknowledgements

The authors express their gratitude to the USDA-CSREES and the Nebraska Corn Board for their support of this work.

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Correspondence to H. Noureddini.

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Noureddini, H., Dang, J. Degradation of Phytates in Distillers’ Grains and Corn Gluten Feed by Aspergillus niger Phytase. Appl Biochem Biotechnol 159, 11–23 (2009). https://doi.org/10.1007/s12010-008-8365-2

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  • DOI: https://doi.org/10.1007/s12010-008-8365-2

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