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Expression of Recombinant Human Nerve Growth Factor Beta in Milk of Goats by Recombinant Replication-Defective Adenovirus

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Abstract

For producing a large quantity of human nerve growth factor beta (hNGF-β), the recombinant adenovirus containing an expression cassette of hNGF-β complementary DNA gene was constructed and then instilled directly into goats’ mammary glands. The recombinant hNGF-β was detected in the milk. The maximal expression level of recombinant hNGF-β in the milk reached 196.8 mg/l. The biological activity of the recombinant hNGF-β, compared with the commercial hNGF-β, was tested using PC12 cells. The results demonstrate that instilling recombinant adenovirus directly into the mammary gland of goat is an efficient approach to producing a large quantity of hNGF-β.

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Acknowledgments

This work was supported by the Science-Technology Investment Company of Qinhuangdao, Hebei, China.

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Authors and Affiliations

  1. College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, People’s Republic of China

    Bo Xiao, Qingwang Li, Bo Feng, Xubo Zhi, Hai Yang & Zhibin Liu

  2. Department of biological Engineering, College of Environment & Chemical Engineering, Yanshan University, Qinhuangdao, 066004, Hebei, People’s Republic of China

    Qingwang Li, Zengsheng Han, Dawei Gao, Rui Zhao, Jian Li & Kun Li

  3. College of Life Sciences, LuDong University, Yantai, 264025, Shandong, People’s Republic of China

    Bo Xiao

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  1. Bo Xiao
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Correspondence to Qingwang Li.

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Xiao, B., Li, Q., Feng, B. et al. Expression of Recombinant Human Nerve Growth Factor Beta in Milk of Goats by Recombinant Replication-Defective Adenovirus. Appl Biochem Biotechnol 157, 357–366 (2009). https://doi.org/10.1007/s12010-008-8346-5

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  • DOI: https://doi.org/10.1007/s12010-008-8346-5

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