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Strain Construction for Ethanol Production from Dilute-Acid Lignocellulosic Hydrolysate

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Abstract

In order to construct a strain that converts sugar mixture and resist/metabolize inhibitors in lignocellulosic dilute-acid hydrolysate, the biotechnology of inactive intergeneric fusion between Saccharomyces cerevisiae and Pachysolen tannophilis was performed. Fusant 1 was successfully obtained as a hybrid strain, which was screened out by xylose and mixed sugar (xylose and glucose) fermentation. This strain showed good abilities of ethanol production, ethanol tolerance, and resistance to the toxic inhibitors presenting in the hydrolysate. The maximum volumetric yield of ethanol and yield of xylitol in mixed sugar was 9.52 g/l and 0.44 g/g, respectively. The results indicated that the constructed strain Fusant 1 was a good producer for ethanol and xylitol from lignocellulosic dilute-acid hydrolysate.

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Acknowledgments

We would like to thank the Ministry of Science and Technology for financial support (nos. 2001AA514024 and 2002AA514010). We gratefully acknowledge the Department of Energy Chemical Engineering (East China University of Science and Technology, Shanghai, China) for providing the hydrolysate used in the experiment.

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Authors and Affiliations

  1. College of Life Science, Capital Normal University, No 105 North Street of West Third Ring Road, Beijing, 100048, China

    Fei Yan, Fali Bai, Shen Tian, Jinxin Zhang, Zuoyang Zhang & Xiushan Yang

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  1. Fei Yan
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  2. Fali Bai
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  3. Shen Tian
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  4. Jinxin Zhang
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  5. Zuoyang Zhang
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  6. Xiushan Yang
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Correspondence to Xiushan Yang.

Additional information

F. Yan, F. Bai, and S. Tian contributed equally to this research work.

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Yan, F., Bai, F., Tian, S. et al. Strain Construction for Ethanol Production from Dilute-Acid Lignocellulosic Hydrolysate. Appl Biochem Biotechnol 157, 473–482 (2009). https://doi.org/10.1007/s12010-008-8343-8

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  • DOI: https://doi.org/10.1007/s12010-008-8343-8

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